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. 2013 Mar;5(1):42-7.

Prevalence and antibiotic resistance patterns of diarrheagenic Escherichia coli isolated from adolescents and adults in Hamedan, Western Iran

Mohammad Yousef Alikhani  1 , Seyyed Hamid Hashemi, Mohammad Mehdi Aslani, Safar Farajnia
Affiliations

Prevalence and antibiotic resistance patterns of diarrheagenic Escherichia coli isolated from adolescents and adults in Hamedan, Western Iran

Mohammad Yousef Alikhani et al. Iran J Microbiol. 2013 Mar.

Abstract

Background and objectives: Pathogenic strains of Escherichia coli are a common cause of acute infectious diarrhea. The aim of this study was to investigate the frequency, virulence markers and antibiotic resistance patterns of diarrheagenic E. coli (DEC) isolated from adolescents and adults in Hamadan, west of Iran.

Materials and methods: A total of 187 stool samples were collected from adults with acute diarrhea. Stool culture was performed by conventional methods for enteropathogenic bacteria. Virulence factor genes for DEC were detected by polymerase chain reaction. Antimicrobial susceptibility was tested using the disk diffusion method.

Results: Among the 187 patients, 40 (21.4%) were positive for DEC. The most frequently identified DEC was enteropathogenic E. coli (47.5%), followed by enteroaggregative (20%), enterotoxigenic (17.5%) and shiga-toxin producing E. coli (15%). No isolates of enteroinvasive E. coli were detected. All STEC strains were stx+ / eaeA-. Out of the seven ETEC strains, five (71.4%) produced ST, one (14.3%) produced only LT and one (14.3%) of the isolates produced both ST and LT encoded by est and elt genes, respectively. Among the 40 DEC strains 27(67.5%) were multidrug resistant.

Conclusion: DEC contribute to the burden of diarrhea in adults in Hamadan. Enteropathogenic E. coli was the most commonly identified DEC strain in the region studied.

Keywords: Antimicrobial resistance; Diarrhea; Escherichia coli; Hamadan.

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References

    1. Kosek M, Bern C, Guerrant RL. The global burden of diarrhoeal disease, as estimated from studies published between 1992 and 2000. Bull World Health Organ. 2003;81:197–204. - PMC - PubMed
    1. Okeke IN, Lamikanra A, Czeczulin J, Dubovsky F, Kaper J, Nataro J. Heterogeneous virulence of enteroaggregative Escherichia coli strains isolated from children in Southwest Nigeria. J Infect Dis. 2000;181:252–260. - PubMed
    1. Presterl E, Zwick RH, Reichmann S, Aichelburg A, Winkler S, Kremsner PG, et al. Frequency and virulence properties of diarrheagenic Escherichia coli in children with diarrhea in Gabon. Am J Trop Med Hyg. 2003;69:406–410. - PubMed
    1. Aslani MM, Alikhani MY. Serotypes of Enteropathogenic Escherichia coli Isolated from Children Under 5 Years of Age. Iranian J Publ Health. 2009;38:70–77.
    1. Alikhani MY, Masoumi Asl H, Khairkhah M, Farajnia S, Aslani MM. Phenotypic and genotypic characterization of Escherichia Coli O111serotypes. Gastroenterol Hepatol From Bed to Bench. 2011;4:147–152. - PMC - PubMed

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