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. 1990 Jun;9(6):1929-37.
doi: 10.1002/j.1460-2075.1990.tb08320.x.

Substrate recognition by RNase P and by the catalytic M1 RNA: identification of possible contact points in pre-tRNAs

D Kahle  1 , U Wehmeyer, G Krupp
Affiliations

Substrate recognition by RNase P and by the catalytic M1 RNA: identification of possible contact points in pre-tRNAs

D Kahle et al. EMBO J. 1990 Jun.

Abstract

Modified bases were introduced into pre-tRNAs during in vitro RNA synthesis or by chemical modification. These RNAs were used as substrates for the catalytic M1 RNA and the RNase P holoenzyme from Schizosaccharomyces pombe. The synthetic approach permitted the insertion of 100% m7GTP into pre-tRNAs and this resulted in complete inhibition of the specific 5' processing reactions. Partially modified RNAs were obtained by chemical modifications of purines and uridines in the pre-tRNAs. This allowed detailed analyses of specific bases excluded in the products. With pre-tRNA(Ser) and initiator pre-tRNA(Met), strong effects were observed in the T arm and weaker effects in the anticodon stem. Only minor base exclusions were detected in the acceptor stem of pre-tRNA(Ser) and in the D arm of pre-tRNA(Met).

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