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. 2013 Mar 20;135(11):4191-4.
doi: 10.1021/ja312039k. Epub 2013 Mar 11.

A homogeneous chemiluminescent immunoassay method

Hashem Akhavan-Tafti  1 , Dean G Binger, John J Blackwood, Ying Chen, Richard S Creager, Renuka de Silva, Robert A Eickholt, Jose E Gaibor, Richard S Handley, Kenneth P Kapsner, Senja K Lopac, Michael E Mazelis, Terri L McLernon, James D Mendoza, Bruce H Odegaard, Sarada G Reddy, Michael Salvati, Barry A Schoenfelner, Nir Shapir, Katherine R Shelly, Jeff C Todtleben, Guoping Wang, Wenhua Xie
Affiliations

A homogeneous chemiluminescent immunoassay method

Hashem Akhavan-Tafti et al. J Am Chem Soc. .

Abstract

A new homogeneous chemiluminescent immunoassay method featuring the use of specific binding members separately labeled with an acridan-based chemiluminescent compound and a peroxidase is reported. Formation of an immunocomplex brings the chemiluminescent compound and the peroxidase into close proximity. Without any separation steps, a chemiluminescent signal is generated upon addition of a trigger solution, and the intensity is directly correlated to the quantity of the analyte.

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