Canine osteosarcoma cell lines from patients with differing serum alkaline phosphatase concentrations display no behavioural differences in vitro

Abstract

Osteosarcoma is an aggressive malignancy and represents the most frequent primary bone malignancy of dogs and humans. Prognostic factors reported for osteosarcoma include tumour size, presence of metastatic disease and serum alkaline phosphatase (ALP) concentration at the time of diagnosis. To date, there have been no studies to determine whether the behaviour of osteosarcoma cells differ based on serum ALP concentration. Here, we report on the generation of six canine osteosarcoma cell lines from osteosarcoma-bearing dogs with differences in serum ALP concentration. To determine whether in vitro behaviour differs between primary osteosarcoma cell lines generated from patients with normal or increased serum ALP, assays were performed to evaluate proliferation, migration, invasion and chemosensitivity. There were no significant differences in cell proliferation, migration, invasion or chemosensitivity between cell lines associated with normal or increased serum ALP concentration.

Keywords: comparative oncology; in vitro models; oncology; small animal; tumour biology.

Figure 1. Characteristics of canine primary OSA cell lines associated with normal or increased serum ALP

There were no differences between primary OSA cell lines associated with normal or increased serum ALP concentration in: (A) ALP activity, (B) cell proliferation, (C) migration, or (D) invasion. Comparisons made using a two-tailed unpaired t-test.

Figure 2. Evaluation of sensitivity of canine primary OSA cell lines associated with normal or increased serum ALP to carboplatin chemotherapy

There is no difference in sensitivity to increasing carboplatin concentrations (0.1–1,000μM) between canine primary OSA cell lines associated with normal or increased serum ALP concentration under: (A) 10% FBS-containing media conditions, or (B) 1% FBS-containing media conditions. Comparisons made using two-way ANOVA testing.

Figure 3. Evaluation of sensitivity of canine primary OSA cell lines associated with normal or increased serum ALP to doxorubicin chemotherapy

There is no difference in sensitivity to increasing doxorubicin concentrations (0.01–1μM) between canine primary OSA cell lines associated with normal or increased serum ALP concentration under: (A) 10% FBS-containing media conditions, or (B) 1% FBS-containing media conditions. Comparisons made using two-way ANOVA testing.