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. 2012 Jan;15(1):683-90.

Mutation Analysis of TP53 Tumor Suppressor Gene in Colorectal Cancer in Patients from Iran (Kerman Province)

Azadeh Lohrasbi Nejad  1 , Mohammad Mehdi Yaghoobi
Affiliations

Mutation Analysis of TP53 Tumor Suppressor Gene in Colorectal Cancer in Patients from Iran (Kerman Province)

Azadeh Lohrasbi Nejad et al. Iran J Basic Med Sci. 2012 Jan.

Abstract

Objectives: P53 is an important tumor suppressor, which is mutated in later stages of many cancers and leads to resistance to chemotherapy. The aim of this study was to reveal mutations of TP53 in colorectal cancer in Kerman province.

Materials and methods: A total of Forty-three colon cancer specimens as paraffin block or fresh tissues, which passed stage IIIA, were selected. Three exons 5, 7 and 8 of P53 were amplified by PCR technique and sequenced directly.

Results: The results showed two deletions at codon 140 and 142 in one tumor sample. GAT→AAT mutation at codon 184, and CGG→TGG mutation at codon 248 were seen in some tumor samples. Some mutations were also observed in middle of intron 7 in some tumor or normal tissues.

Conclusion: Some of those patients with mutation in P53 gene had metastasis in other organs. Therefore, genetic test before chemotherapy is helpful for successful treatment.

Keywords: Colorectal cancer; Point mutation; tp35 tumor suppressor gene.

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Figures

Figure 1
Figure 1
PCR products amplified by Taq Polymerase (1, 2) and pwo (3, 4) on agarose gel electrophoresis. NC=Negative control, PC=Positive control. As it is obvious the exon 8 segment is substantially amplified by pwo while weak band is seen when Taq polymerase is used in PCR reaction.
Figure 2
Figure 2
Sequencing result of tumor DNA of a patient shows deletion at codon 140 and 142. Normal tissue of the patient has normal sequence.
Figure 3
Figure 3
Sequencing of a tumor DNA shows GAT→AAT conversion at codon 184.
Figure 4
Figure 4
Sequencing result of two tumor DNAs show CGG→TGG conversion at codon 248.
Figure 5
Figure 5
Insertion of G and substitution of T insteead of C in the middle of intron 7-8.
See this image and copyright information in PMC

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