Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2007 Fall;6(1):A1-7.
Epub 2007 Oct 15.

DataView: A Tutorial Tool for Data Analysis. Template-based Spike Sorting and Frequency Analysis

William J Heitler  1
Affiliations

DataView: A Tutorial Tool for Data Analysis. Template-based Spike Sorting and Frequency Analysis

William J Heitler. J Undergrad Neurosci Educ. 2007 Fall.

Abstract

DataView is a Windows program for viewing and analyzing digital data derived from analog signals using A/D acquisition systems. It is primarily designed for research neuroscientists, but its low (or zero) cost makes it a suitable tool for giving students hands-on experience of analysis techniques. It is supplied with many examples of pre-recorded data and also has facilities to allow tutors to use their own - or indeed their students' - experimental results as the dataset for analysis. This article illustrates the use of DataView with a brief tutorial on template-based spike sorting and frequency analysis. The DataView manual contains detailed instructions and sample datasets for a wide range of other analytical and data-manipulation techniques.

Keywords: DataView; caudal photoreceptor, crayfish, spike sorting, frequency analysis; digital data analysis.

PubMed Disclaimer

Figures

Figure 1.
Figure 1.
A screen shot of DataView displaying the response of the crayfish ventral nerve cord, including the caudal photoreceptor, to a light stimulus.
Figure 2.
Figure 2.
An expanded view showing three spikes which are potential candidates as templates for use in CPR spike recognition. The window showing the whole record has been minimized.
Figure 3.
Figure 3.
A scattergraph of time versus instantaneous frequency for events which match the template with an allowed error which has been set deliberately high.
Figure 4.
Figure 4.
A histogram showing the distribution of errors of waveforms that match the template within an allowed error of 25. Note that there is a single event with an error of 0 (the template itself) and that there is a discontinuity in the histogram at an error value of about 10.
Figure 5.
Figure 5.
A scattergraph of time versus instantaneous frequency for events which closely match a template derived by averaging the waveforms matching an earlier template which was itself derived from a single spike which probably represented a genuine CPR spike.
Figure 6.
Figure 6.
A spike pair which match the template, but which produce a high frequency outlier. One of the pair must be a false positive.
Figure 7.
Figure 7.
Spike overlap distorts the waveform of a CPR spike in the center of the display, causing a false negative and a half-frequency outlier. The position of the trace of event channel b has been dragged below the spike waveform for clarity.
Figure 8.
Figure 8.
Superimposed images of the averaged waveforms of two spikes, the strongly photosensitive CPR spike, and a larger very weakly photosensitive non-CPR spike.
See this image and copyright information in PMC

References

    1. George S. Data acquisition and display for electrophysiology: PC oscilloscopes. J Undergrad Neurosci Ed. 2006;5:R11–R14. - PMC - PubMed
    1. Harris KD. 2000–2002. http://klustakwik.sourceforge.net.
    1. Hoy RR, Johnson BR, Wyttenbach RA. Crawdad: A CD-ROM lab manual for neurophysiology. Sunderland, MA: Sinauer; 2002.
    1. Lewicki MS. A review of methods of spike sorting: The detection and classification of neural action potentials. Network: Comput Neural Syst. 1998;9:R53–R78. - PubMed
    1. Prosser CL. Action potentials in the nervous system of the crayfish. II. Responses to illumination of the eye and caudal ganglion. J Cell Comp Physiol. 1934;4:363–377.

LinkOut - more resources