doi: 10.1007/978-1-62703-302-2_15.
Isolation of Dictyostelium nuclei for light and electron microscopy
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- PMID: 23494313
- DOI: 10.1007/978-1-62703-302-2_15
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Isolation of Dictyostelium nuclei for light and electron microscopy
Methods Mol Biol.
2013.
Abstract
The significance of amoebae for studies of nuclear architecture has considerably increased in the recent years. The availability of a protocol for isolation of nuclei in a quality sufficient for high-resolution light and electron microscopy is a prerequisite for such studies. Here we present a protocol for high enrichment of nuclei by sucrose density-gradient centrifugation. Moreover, we describe how to use these isolated nuclei as specimens for immunofluorescence and immune-electron microscopy of ultrathin sections.
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