Ceramicine B, a limonoid with anti-lipid droplets accumulation activity from Chisocheton ceramicus

Abstract

The Meliaceae family of plants has been shown to contain a vast number of compounds with the potential to be developed for medicinal purposes. We have previously reported the isolation of limonoids from a plant in the Meliaceae family named Chisocheton ceramicus. Ceramicine B was identified as an active compound in inhibiting lipid droplets accumulation (LDA) in the mouse preadipocyte cell line MC3T3-G2/PA6. The presence of ceramicine B was found to inhibit the expression of glucose transporter type 4, lipoprotein lipase, and 11-beta hydroxysteroid dehydrogenase mRNA, and also adipogenic master regulator, peroxisome proliferator-activated receptor-γ, and CCAAT-enhancer-binding protein-α (C/EBPα) mRNA. However, for early adipogenic regulators, such as C/EBPβ and C/EBPδ, and intermediary adipogenic regulators, Krüppel-like factors were unaffected. Western blot analysis showed that ceramicine B was found to inhibit the phosphorylation of Forkhead box O1 (Foxo1), a key process in the insulin signaling pathway. This suggested that the mechanism of anti-LDA activity of ceramicine B was partly via the inhibition of Foxo1 phosphorylation.