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. 2013 Feb 1;2(1):3-7.
doi: 10.1016/j.coche.2012.11.002.

Stem Cell Separation Technologies

Beili Zhu  1 , Shashi K Murthy
Affiliations

Stem Cell Separation Technologies

Beili Zhu et al. Curr Opin Chem Eng. .

Abstract

Stem cell therapy and translational stem cell research require large-scale supply of stem cells at high purity and viability, thus leading to the development of stem cell separation technologies. This review covers key technologies being applied to stem cell separation, and also highlights exciting new approaches in this field. First, we will cover conventional separation methods that are commercially available and have been widely adapted. These methods include Fluorescence-activated cell sorting (FACS), Magnet-activated cell sorting (MACS), pre-plating, conditioned expansion media, density gradient centrifugation, field flow fractionation (FFF), and dielectrophoresis (DEP). Next, we will introduce emerging novel methods that are currently under development. These methods include improved aqueous two-phase system, systematic evolution of ligands by exponential enrichment (SELEX), and various types of microfluidic platforms. Finally, we will discuss the challenges and directions towards future breakthroughs for stem cell isolation. Advancing stem cell separation techniques will be essential for clinical and research applications of stem cells.

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References

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    1. Gothard D, et al. In search of the skeletal stem cell: isolation and separation strategies at the macro/micro scale for skeletal regeneration. Lab Chip. 2011;11(7):1206–1220. * Provides a good review on the field of cell separation for the skeletal muscle tissue engineering. Many of the separation techniques translate over to other applications as well.