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. 2013 Feb;5(1):2-8.
doi: 10.4047/jap.2013.5.1.2. Epub 2013 Feb 28.

Sterilization effect of atmospheric pressure non-thermal air plasma on dental instruments

Su-Jin Sung  1 , Jung-Bo Huh, Mi-Jung Yun, Brian Myung W Chang, Chang-Mo Jeong, Young-Chan Jeon
Affiliations

Sterilization effect of atmospheric pressure non-thermal air plasma on dental instruments

Su-Jin Sung et al. J Adv Prosthodont. 2013 Feb.

Abstract

Purpose: Autoclaves and UV sterilizers have been commonly used to prevent cross-infections between dental patients and dental instruments or materials contaminated by saliva and blood. To develop a dental sterilizer which can sterilize most materials, such as metals, rubbers, and plastics, the sterilization effect of an atmospheric pressure non-thermal air plasma device was evaluated.

Materials and methods: After inoculating E. coli and B. subtilis the diamond burs and polyvinyl siloxane materials were sterilized by exposing them to the plasma for different lengths of time (30, 60, 90, 120, 180 and, 240 seconds). The diamond burs and polyvinyl siloxane materials were immersed in PBS solutions, cultured on agar plates and quantified by counting the colony forming units. The data were analyzed using one-way ANOVA and significance was assessed by the LSD post hoc test (α=0.05).

Results: The device was effective in killing E. coli contained in the plasma device compared with the UV sterilizer. The atmospheric pressure non-thermal air plasma device contributed greatly to the sterilization of diamond burs and polyvinyl siloxane materials inoculated with E. coli and B. subtilis. Diamond burs and polyvinyl siloxane materials inoculated with E. coli was effective after 60 and 90 seconds. The diamond burs and polyvinyl siloxane materials inoculated with B. subtilis was effective after 120 and 180 seconds.

Conclusion: The atmospheric pressure non-thermal air plasma device was effective in killing both E. coli and B. subtilis, and was more effective in killing E. coli than the UV sterilizer.

Keywords: Bacteria; Cross Infections; Non-thermal Atmospheric Pressure Plasma; Sterilization.

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Figures

Fig. 1
Fig. 1
Schematic view of an atmospheric pressure non-thermal air plasma device. A: The frontal view, B: The rear view.
Fig. 2
Fig. 2
Schematic view of a UV sterilizer.
Fig. 3
Fig. 3
Inoculated material. A: Diamond bur, B: Polyvinyl siloxane.
Fig. 4
Fig. 4
Survival curve (CFU/mL) of E. coli after treatment with an atmospheric pressure non-thermal air plasma device as a function of treatment time.
Fig. 5
Fig. 5
Survival curve (CFU/mL) of B. subtilis after treatment with an atmospheric pressure non-thermal air plasma device as a function of treatment time.
See this image and copyright information in PMC

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