Cache valley virus in a patient diagnosed with aseptic meningitis

Abstract

Cache Valley virus was initially isolated from mosquitoes and had been linked to central nervous system-associated diseases. A case of Cache Valley virus infection is described. The virus was cultured from a patient's cerebrospinal fluid and identified with real-time reverse transcription-PCR and sequencing, which also yielded the complete viral coding sequences.

Fig 1

Cytopathic effect of the CVV-infected Vero cells. Vero cells were seeded in a 24-well plate and grown to 90% confluence and were either mock infected (A) or infected at a multiplicity of infection (MOI) of 0.3 (B) or at an MOI of 30 (C) with the MNZ-92011 strain, which was harvested from BGMK cells, amplified, and titrated in Vero cells. Photographs were taken on day 4 postinfection, using a Spot RT-KE monochrome camera connected to a Nikon Diaphot TMD microscope at ×40 magnification and Spot 5.0 Basic software.

Fig 2

Detection of anti-CVV antibodies in the patient's convalescent-phase serum. The results of an IFA of the patient's 24-day convalescent-phase serum (I), an anti-CVV mouse hyperimmune serum (II), and an anti-La Crosse virus human convalescent-phase serum (III), using Caco2 cells that were mock infected (BI to BIII) or at day 2 postinfection with the MNZ-92011 strain (AI to AIII), are shown. Fluorescein isothiocyanate (FITC)-conjugated dual anti-human/anti-mouse IgG goat antibody (Focus Diagnostics, Cypress, CA) was used as a secondary antibody. Images were taken with a Zeiss AxioCam MRc camera attached to AxioImager A1 microscope at ×400 magnification, using Zeiss AxioVision 3.1 software. All sera were at a dilution of 1:16, with the exception of the anti-CVV mouse serum represented in panels AII and BII, where the serum was diluted 1:1,024.