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Comparative Study
. 2013 Jun;51(6):1913-6.
doi: 10.1128/JCM.00661-13. Epub 2013 Mar 20.

Performance of two commercial assays for detecting hepatitis E virus RNA in acute or chronic infections

Florence Abravanel  1 , Sabine Chapuy-Regaud, Sébastien Lhomme, Martine Dubois, Jean-Marie Peron, Laurent Alric, Lionel Rostaing, Nassim Kamar, Jacques Izopet
Affiliations
Comparative Study

Performance of two commercial assays for detecting hepatitis E virus RNA in acute or chronic infections

Florence Abravanel et al. J Clin Microbiol. 2013 Jun.

Abstract

We assessed the performance of the Ceeram and Altona assays, the first two commercially available hepatitis E virus (HEV) RNA assays, using serial dilutions of 4 HEV-positive reference samples (genotypes 3a, 3c, 3e, and 3f). Both assays provided good analytical sensitivity and high reproducibility for detecting genotype 3 HEV RNA.

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Figures

Fig 1
Fig 1
Standard curves generated using dilutions of the WHO reference standard. (A) Ceeram assay. (B) Altona assay. Data are means of three replicates for each standard dilution.
Fig 2
Fig 2
Correlation between CT values obtained with the Ceeram and Altona assays for the 4 reference strains.
See this image and copyright information in PMC

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