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. 1990;4(3):175-9.
doi: 10.1002/jcla.1860040305.

Multiparametric analysis of peripheral blood in the normal pediatric population by flow cytometry

D Wiener  1 , S Shah, J Malone, N Lowell, S Lowitt, D T Rowlands Jr
Affiliations

Multiparametric analysis of peripheral blood in the normal pediatric population by flow cytometry

D Wiener et al. J Clin Lab Anal. 1990.

Abstract

Lymphocyte subset analysis was performed on 114 healthy children and 84 healthy adults. Samples were prepared by a whole blood lysis technique and analyzed by flow cytometry. The percentage and total number of CD3+, CD4+, CD8+, and CD19+ lymphocytes were calculated for each of six age groups. A direct correlation with age was seen in the percentages of CD3+, CD4+, and CD8+ lymphocytes. The absolute number and percentage of total lymphocytes, the percentage and absolute number of CD19+ lymphocytes, and the absolute number of CD3+ lymphocytes decreased with age. No significant correlation with age was observed for white blood cells, the absolute number of CD4+ and CD8+ lymphocytes, and the CD4+/CD8+ ratio.

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