doi: 10.1002/cbic.201200762.
Epub 2013 Mar 25.
The mechanisms of radical SAM/cobalamin methylations: an evolving working hypothesis
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- PMID: 23526745
- DOI: 10.1002/cbic.201200762
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The mechanisms of radical SAM/cobalamin methylations: an evolving working hypothesis
Chembiochem.
.
Abstract
ALL ABOUT ME: Pierre and co-workers have revealed mechanistic details of a tryptophan methyltransferase (TsrM) involved in the biosynthesis of the thiopeptide antibiotic, thiostrepton. Utilising cobalamin and a [4Fe-4S] cluster to generate 2-methyltryptophan from tryptophan, a key difference between this enzyme and other radical SAM methyltransferases is that the reaction is not initiated by a single-electron reduction of SAM to generate 5'-dA⋅.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
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