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. 2013:2013:103960.
doi: 10.1155/2013/103960. Epub 2013 Feb 26.

Production of Enzymes from Agroindustrial Wastes by Biosurfactant-Producing Strains of Bacillus subtilis

Francisco Fábio Cavalcante Barros  1 , Ana Paula Resende Simiqueli, Cristiano José de Andrade, Gláucia Maria Pastore
Affiliations

Production of Enzymes from Agroindustrial Wastes by Biosurfactant-Producing Strains of Bacillus subtilis

Francisco Fábio Cavalcante Barros et al. Biotechnol Res Int. 2013.

Abstract

Bacteria in the genus Bacillus are the source of several enzymes of current industrial interest. Hydrolases, such as amylases, proteases, and lipases, are the main enzymes consumed worldwide and have applications in a wide range of products and industrial processes. Fermentation processes by Bacillus subtilis using cassava wastewater as a substrate are reported in the technical literature; however, the same combination of microorganisms and this culture medium is limited or nonexistent. In this paper, the amylase, protease, and lipase production of ten Bacillus subtilis strains previously identified as biosurfactant producers in cassava wastewater was evaluated. The LB1a and LB5a strains were selected for analysis using a synthetic medium and cassava wastewater and were identified as good enzyme producers, especially of amylases and proteases. In addition, the enzymatic activity results indicate that cassava wastewater was better than the synthetic medium for the induction of these enzymes.

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Figures

Figure 1
Figure 1
Average halo diameter in plates for LB1a and LB5a Bacillus subtilis strains: (a) amylase, (b) protease, and (c) lipase.
Figure 2
Figure 2
Milk agar plates after 24 h (a) and 72 h (b) inoculated with Bacillus subtilis strains LB117 and LB1a (a) and LB5a and ATCC21332 (b); the proteolysis halos are evident.
Figure 3
Figure 3
Enzymatic activity kinetics of LB5a and LB1a strains in a synthetic medium supplemented: (a) amylase, (b) protease, and (c) lipase.
Figure 4
Figure 4
Enzymatic activity kinetics of LB5a and LB1a strains grown in glass flasks using cassava wastewater as culture medium: (a) amylase, (b) protease, and (c) lipase.
Figure 5
Figure 5
Enzymatic activity kinetics of LB5a and LB1a strains grown in bioreactor using cassava wastewater as culture medium: (a) amylase, (b) protease, and (c) lipase.
See this image and copyright information in PMC

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