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. 1990;20(4):715-24.

Steady state fluorescence polarization and Fourier transform infrared spectroscopy studies on membranes of functionally senescent human erythrocytes

R Fiorini  1 , G Curatola, A Kantar, P L Giorgi, E Bertoli, F Tanfani
Affiliations
  • PMID: 2353921

Steady state fluorescence polarization and Fourier transform infrared spectroscopy studies on membranes of functionally senescent human erythrocytes

R Fiorini et al. Biochem Int. 1990.

Abstract

Human erythrocytes were separated using Percoll/Albumin density gradient centrifugation into 5 subpopulations which represent cell subpopulations of different ages. The physico-chemical properties of the membranes and their lipid extracts, prepared from the 5 subpopulations, were studied by 1,6-diphenyl-1,3,5-hexatriene fluorescence polarization and by Fourier Transform Infrared Spectroscopy respectively. The fluorescence polarization data show a decrease of membrane fluidity in the youngest erythrocyte fraction and the infrared spectroscopy reveals a lower degree of unsaturation of the lipids from this fraction. Changes of membrane fluidity among the 5 erythrocyte subpopulations is assigned to the variations of the degree of unsaturations of lipids rather than to variations of cholesterol content.

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