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Case Reports
. 2013 Dec;2(1):106.
doi: 10.1186/2193-1801-2-106. Epub 2013 Mar 12.

Utility of two novel multiplexing assays for the detection of gastrointestinal pathogens - a first experience

Pascal Kahlau  1 , Monika Malecki, Verena Schildgen, Christine Schulz, Ingo Winterfeld, Sabine Messler, Frauke Mattner, Oliver Schildgen
Affiliations
Case Reports

Utility of two novel multiplexing assays for the detection of gastrointestinal pathogens - a first experience

Pascal Kahlau et al. Springerplus. 2013 Dec.

Abstract

Background: Cause for gastroenteritis range from viral, bacterial to parasitic pathogens. Rapid Multiplexing techniques like ProGastro_SSCS and xTAG_GPP can detect broad panels of pathogens simultaneously. We performed a field test with a total number of 347 stool samples from adult hospitalized patients that were tested with the Luminex xTAG GPP assay; of the 157 samples positively tested for at least one pathogen by xTAG GPP a total number of 30 samples was retested with the ProGastro SSCS assay. Assays were compared to standard routine diagnostics.

Findings: Multiplexing significantly reduced the time to the initial identification of a pathogen. Moreover, multiplexing detected pathogens for which a diagnostic assays was not requested by the physician and thus may be an important tool for avoiding nosocomial outbreaks.

Conclusion: This first frontline approach with these assays approves their utility compared to conventional microbiological methods.

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Figures

Figure 1
Figure 1
Time needed from receiving a sample to communicate the diagnostic findings. The 104 samples handled with the xTAG GPP were compared to the corresponding samples that were tested with conventional methods. For reasons of comparability the median (xTAG GPP: green, conventional: red) was drawn in the diagram.
See this image and copyright information in PMC

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