[Molecular detection of hepatitis E virus in pig livers destined for human consumption in the state of Nuevo Leon, Mexico]
- PMID: 23546411
- DOI: 10.1590/s0036-36342013000200011
[Molecular detection of hepatitis E virus in pig livers destined for human consumption in the state of Nuevo Leon, Mexico]
Abstract
Objective: Molecular detection of HEV in pig livers destined for human consumption in Nuevo Leon, Mexico.
Materials and methods: 87 livers were collected from pigs slaughtered in TIF and 40 livers from butchers. A 212 pb fragment of HEV ORF2 gene was amplified by semi-nested RT-PCR.
Results: 19.54% (17) of tif's and 22.5% (9) of butcher's livers were positive for HEV. Sequencing of the amplified products showed a 94%-95% homology with the sequences reported for genotype 3.
Conclusions: Our results indicate that HEV is circulating in swine herds in the state, constituting a probable source of contamination of pig meat products.
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