doi: 10.1002/0471142727.mb0214s102.
DNA isolation from mammalian samples
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- PMID: 23547013
- DOI: 10.1002/0471142727.mb0214s102
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DNA isolation from mammalian samples
Curr Protoc Mol Biol.
2013 Apr.
Abstract
DNA isolation is a fundamental initial step for molecular genetic studies. Two quite different methodologies are described in this unit: silica spin column and phenol/chloroform extraction. Currently, the most commonly used technique is the silica-based spin column, although phenol/chloroform extraction is still widely used. This unit also presents basic procedures for extraction of DNA from both fresh tissues and formalin-fixed, paraffin-embedded (FFPE) tissue samples.
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