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. 2013 Jul;208(1):101-8.
doi: 10.1093/infdis/jit139. Epub 2013 Apr 1.

The immune response to pneumococcal polysaccharides 14 and 23F among elderly individuals consists predominantly of switched memory B cells

David J Leggat  1 , Rebecca S Thompson, Noor M Khaskhely, Anita S Iyer, M A Julie Westerink
Affiliations

The immune response to pneumococcal polysaccharides 14 and 23F among elderly individuals consists predominantly of switched memory B cells

David J Leggat et al. J Infect Dis. 2013 Jul.

Abstract

The phenotype of B cells that respond to vaccination with the purified pneumococcal polysaccharide (PPS) has been a topic of debate. We have recently identified the phenotype of cells from healthy young volunteers as CD27(+)IgM(+) B cells. However, the PPS-responding B-cell population has not yet been identified in high-risk populations, such as elderly individuals. Previous studies have shown that elderly individuals have a lower percentage of immunoglobulin M memory B cells than healthy young adults. In this study, we directly characterized the phenotype of PPS-specific B cells before and after vaccination with PPS vaccine (PPV) in elderly adults, using fluorescently labeled PPS14 and PPS23F. In contrast to our observations in healthy young volunteers, the PPS-responding B-cell population consisted primarily of switched memory (CD27(+)IgM(-)) B cells. In concurrence with these findings, postvaccination immunoglobulin M concentrations were not significantly increased in this population, and the opsonophagocytic response was decreased, compared with that in young adults. These findings identify a significant shift in the phenotype of the B-cell population in response to PPV among elderly individuals.

Keywords: 23F; B cell; CD27; Pneumococcus; Streptococcus pneumoniae; elderly; flow cytometry; human; polysaccharide; vaccine.

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Figures

Figure 1.
Figure 1.
Pre- and postimmunization pneumococcal polysaccharide (PPS)–selected B-cell phenotypes in elderly individuals. The phenotypes of B lymphocytes that respond to vaccination with PPS vaccine were determined by flow cytometry. Before and 7 days after vaccination, circulating peripheral blood mononuclear cells were isolated and labeled fluorescently with different anti-surface marker antibodies. The phenotype of unselected and PPS14- and PPS23F-selected B cells were compared in elderly individuals. In each sample, 100 000 events were recorded. Abbreviation: IgM, immunoglobulin M
Figure 2.
Figure 2.
Pneumococcal polysaccharide (PPS)–labeled B-cell phenotypes in elderly individuals, compared with young adults. The phenotypes of B lymphocytes that respond to vaccination with PPS vaccine were determined by flow cytometry. Before vaccination and 7 days after vaccination, circulating peripheral blood mononuclear cells were isolated and labeled fluorescently with different anti-surface marker Abs. The phenotype of pre- and postvaccination PPS14-selected (A) and PPS23F-selected (B) B cells was compared in young and elderly individuals. In each sample, 100 000 events were recorded. Abbreviation: IgM, immunoglobulin M.
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References

    1. Recommendations of the immunization practices advisory committee pneumococcal polysaccharide vaccine. MMWR. 1989;38:64–8. 73–6. - PubMed
    1. Jackson LA, Neuzil KM, Yu O, et al. Effectiveness of pneumococcal polysaccharide vaccine in older adults. N Engl J Med. 2003;348:1747–55. - PubMed
    1. Shapiro ED, Berg AT, Austrian R, et al. The protective efficacy of polyvalent pneumococcal polysaccharide vaccine. New Engl J Med. 1991;325:1453–60. - PubMed
    1. Simberkoff MS, Cross AP, Al-Ibrahim M, et al. Efficacy of pneumococcal vaccine in high-risk patients. Results of a Veterans Administration Cooperative Study. N Engl J Med. 1986;315:1318–27. - PubMed
    1. Roghmann KJ, Tabloski PA, Bentley DW, Schiffman G. Immune response of elderly adults to pneumococcus: variation by age, sex, and functional impairment. J Gerontol. 1987;42:265–70. - PubMed

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