Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Case Reports
. 2013 Jun;51(6):2006-7.
doi: 10.1128/JCM.00446-13. Epub 2013 Apr 3.

Identification of an influenza A H1N1/2009 virus with mutations in the matrix gene causing a negative result by a commercial molecular assay

Matthew J Binnicker, Larry M Baddour, Thomas E Grys, Mark J Espy, D Jane Hata, Jason T Wotton, Robin Patel
Case Reports

Identification of an influenza A H1N1/2009 virus with mutations in the matrix gene causing a negative result by a commercial molecular assay

Matthew J Binnicker et al. J Clin Microbiol. 2013 Jun.
No abstract available

PubMed Disclaimer

Figures

Fig 1
Fig 1
Analysis of an influenza A virus 2009/H1N1 strain with point mutations in the matrix gene by real-time PCR (A) and agarose gel electrophoresis (B). The positive control (line and lane 1), patient culture isolate (lines and lanes 2 and 3), and negative control (line and lane 4) were tested by the Prodesse ProFlu+ real-time PCR assay. The PCR product was analyzed in real time for fluorescent output (A) and by agarose gel electrophoresis with ethidium bromide staining (B). Lanes L, molecular weight ladders.
See this image and copyright information in PMC

References

    1. Dhiman N, Espy MJ, Irish C, Wright P, Smith TF, Pritt BS. 2010. Mutability in the matrix gene of novel influenza A H1N1 virus detected using a FRET probe-based real-time reverse transcriptase PCR assay. J. Clin. Microbiol. 48:677–679 - PMC - PubMed
    1. Zheng X, Todd KM, Yen-Lieberman B, Kaul K, Mangold K, Shulman ST. 2010. Unique finding of a 2009 H1N1 influenza virus-positive clinical sample suggests matrix gene sequence variation. J. Clin. Microbiol. 48:665–666 - PMC - PubMed
    1. Novak-Weekly SM, Marlowe EM, Poulter M, Dwyer D, Speers D, Rawlinson W, Baleriola C, Robinson CC. 2012. Evaluation of the Cepheid Xpert Flu assay for rapid identification and differentiation of influenza A, influenza A 2009 H1N1, and influenza B viruses. J. Clin. Microbiol. 50:1704–1710 - PMC - PubMed
    1. Tang Y, Lowery KS, Valsamakis A, Schaefer VC, Chappell JD, White-Abell J, Quinn CD, Li H, Washington CA, Cromwell J, Ciamanco CM, Forman M, Holden J, Rothman RE, Parker ML, Ortenberg EV, Zhang L, Lin Y, Gaydos CA. 2013. Clinical accuracy of a PLEX-ID flu device for simultaneous detection and identification of influenza viruses A and B. J. Clin. Microbiol. 51:40–45 - PMC - PubMed
    1. Zitterkopf NL, Leekha S, Espy MJ, Wood CM, Sampathkumar P, Smith TF. 2006. Relevance of influenza A virus detection by PCR, shell vial assay, and tube cell culture to rapid reporting procedures. J. Clin. Microbiol. 44:3366–3367 - PMC - PubMed

Associated data

LinkOut - more resources