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. 2011 Mar;25(2):141-7.
doi: 10.1016/S1674-8301(11)60018-3.

mRNA profiles of cytokine receptors in unstimulated peripheral blood mononuclear cells from patients with chronic idiopathic urticaria

Jianming Gao  1 , Aizhen Yang, Min Chen, Ansheng Li, Xu Yao, Yumei Li, Shihai Xie, Xueyuan Yang, Liansheng Zhong, Zhiqiang Chen
Affiliations

mRNA profiles of cytokine receptors in unstimulated peripheral blood mononuclear cells from patients with chronic idiopathic urticaria

Jianming Gao et al. J Biomed Res. 2011 Mar.

Abstract

This present study was aimed to investigate the roles of the receptors of Th1/Th2 cytokines and chemokines in the pathogenesis of chronic idiopathic urticaria (CIU). Thirty patients with CIU, 30 patients with dermographism and 30 healthy controls were randomly enrolled. Reverse transcription-PCR (RT-PCR) was used to analyze the mRNA of cytokine receptors in peripheral blood mononuclear cells (PBMCs). The mRNA levels of tumor necrosis factor receptor (TNFR), interferon-γ receptor (IFN-γR), and interleukin-10 receptor (IL-10R) were statistically increased in the CIU group (P < 0.05), while IL-2R, IL-4R, IL-6R, and IL-13R showed no significant differences between the CIU and other groups. The mRNA levels of CCR3 and CCR6 were statistically increased in the CIU group (P < 0.05). The toll-like receptor 2 (TLR2) mRNA level was significantly lower in the CIU group than the healthy control group (P < 0.05). These findings indicate that the regulation of mRNA of TNFR, IFN-γR, IL-10R, CCR3, CCR6 and TLR2 may be involved in the pathogenesis of CIU.

Keywords: chemokine; cytokine; receptor; urticaria.

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Conflict of interest statement

The authors reported no conflict of interests.

Figures

Fig. 1
Fig. 1. Profiles of the mRNA transcripts of cytokines (A) and chemokines (B) in patients with CIU or DG and HC.
The levels of mRNA were examined by RT-PCR. The detection of IFNR, TLR2 and TLR5 mRNA was not conducted in the DG group in A. CIU: chronic idiopathic urticaria; DG: dermographism; HG: healthy control.
See this image and copyright information in PMC

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