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. 2012 Jul;26(4):268-73.
doi: 10.7555/JBR.26.20110121. Epub 2012 Jun 25.

O-linked N-acetylglucosamine transferase (OGT) is overexpressed and promotes O-linked protein glycosylation in esophageal squamous cell carcinoma

Zhe Qiao  1 , Chengxue Dang, Bin Zhou, Shaomin Li, Wei Zhang, Jiantao Jiang, Jin Zhang, Ranran Kong, Yuefeng Ma
Affiliations

O-linked N-acetylglucosamine transferase (OGT) is overexpressed and promotes O-linked protein glycosylation in esophageal squamous cell carcinoma

Zhe Qiao et al. J Biomed Res. 2012 Jul.

Abstract

The aim of this present study was to explore the expression and clinical significance of O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) and enzymatic O-linked glycosylation (O-GlcNAcation) through the addition of O-linked-β-N-acetylglucosamine in esophageal squamous cell carcinoma. OGT expression and O-GlcNAcation in 40 samples from patients with esophageal squamous cell carcinoma was detected by immunohistochemical staining with anti-OGT antib ody and O-GlcNAc-specific antibody RL2, respectively. The relationship between pathological and clinical factors of patients was analyzed. We found that the expression of OGT was higher in esophageal squamous cell carcinoma samples compared to the normal tissues. RL2 antibody level was positively correlated with OGT expression, and the metastasis of lymph node, which means the level of O-GlcNAcation was high and related to the metastasis of lymph node in esophageal squamous cell carcinoma. In conclusion, OGT activation is the main reason for promoting the level of O-GlcNAcation in esophageal squamous cell carcinoma. O-GlcNAcylation may play an important role in esophageal squamous cell carcinoma.

Keywords: O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT); O-linked glycosylation (O-GlcNAcation); esophageal squamous cell carcinoma.

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Conflict of interest statement

The authors reported no conflict of interest.

Figures

Fig. 1
Fig. 1. OGT and RL2 expression in normal and esophageal squamous cell carcinoma (SP staining, ×100).
A: Expression of OGT in normal esophageal mucosa. B: Expression of OGT in esophageal squamous cell carcinoma. C: Expression of O-GlcNAc in normal esophageal mucosa. D: Expression of O-GlcNAc in esophageal squamous cell carcinoma.
See this image and copyright information in PMC

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