Nucleocytoplasmic transport and processing of small nuclear RNA precursors
- PMID: 2355910
- PMCID: PMC360761
- DOI: 10.1128/mcb.10.7.3365-3375.1990
Nucleocytoplasmic transport and processing of small nuclear RNA precursors
Abstract
We have analyzed the structures and locations of small nuclear RNA (snRNA) precursors at various stages in their synthesis and maturation. In the nuclei of pulse-labeled Xenopus laevis oocytes, we detected snRNAs that were longer than their mature forms at their 3' ends by up to 10 nucleotides. Analysis of the 5' caps of these RNAs and pulse-chase experiments showed that these nuclear snRNAs were precursors of the cytoplasmic pre-snRNAs that have been observed in the past. Synthesis of pre-snRNAs was not abolished by wheat germ agglutinin, which inhibits export of the pre-snRNAs from the nucleus, indicating that synthesis of these RNAs is not obligatorily coupled to their export. Newly synthesized U1 RNAs could be exported from the nucleus regardless of the length of the 3' extension, but pre-U1 RNAs that were elongated at their 3' ends by more than about 10 nucleotides were poor substrates for trimming in the cytoplasm. The structure at the 3' end was critical for subsequent transport of the RNA back to the nucleus. This requirement ensures that truncated and incompletely processed U1 RNAs are excluded from the nucleus.
Similar articles
-
Monomethylated cap structures facilitate RNA export from the nucleus.Cell. 1990 Oct 5;63(1):109-18. doi: 10.1016/0092-8674(90)90292-m. Cell. 1990. PMID: 2208274
-
Nuclear processing of the 3'-terminal nucleotides of pre-U1 RNA in Xenopus laevis oocytes.Mol Cell Biol. 1992 Apr;12(4):1553-60. doi: 10.1128/mcb.12.4.1553-1560.1992. Mol Cell Biol. 1992. PMID: 1549111 Free PMC article.
-
Multiple cis-acting signals for export of pre-U1 snRNA from the nucleus.Genes Dev. 1993 Oct;7(10):1898-908. doi: 10.1101/gad.7.10.1898. Genes Dev. 1993. PMID: 8405997
-
Spliceosomal snRNAs, the Essential Players in pre-mRNA Processing in Eukaryotic Nucleus: From Biogenesis to Functions and Spatiotemporal Characteristics.Adv Biol (Weinh). 2024 Jul;8(7):e2400006. doi: 10.1002/adbi.202400006. Epub 2024 May 26. Adv Biol (Weinh). 2024. PMID: 38797893 Review.
-
Pre-mRNA splicing in the nuclei of Xenopus oocytes.Methods Mol Biol. 2006;322:149-63. doi: 10.1007/978-1-59745-000-3_11. Methods Mol Biol. 2006. PMID: 16739722 Review.
Cited by
-
Specific sequence features, recognized by the SMN complex, identify snRNAs and determine their fate as snRNPs.Mol Cell Biol. 2005 Dec;25(24):10989-1004. doi: 10.1128/MCB.25.24.10989-11004.2005. Mol Cell Biol. 2005. PMID: 16314521 Free PMC article.
-
Nuclear export of different classes of RNA is mediated by specific factors.J Cell Biol. 1994 Mar;124(5):627-35. doi: 10.1083/jcb.124.5.627. J Cell Biol. 1994. PMID: 7509815 Free PMC article.
-
Structural requirements of 5S rRNA for nuclear transport, 7S ribonucleoprotein particle assembly, and 60S ribosomal subunit assembly in Xenopus oocytes.Mol Cell Biol. 1993 Nov;13(11):6819-31. doi: 10.1128/mcb.13.11.6819-6831.1993. Mol Cell Biol. 1993. PMID: 8413275 Free PMC article.
-
Involvement of nuclear import and export factors in U8 box C/D snoRNP biogenesis.Mol Cell Biol. 2007 Oct;27(20):7018-27. doi: 10.1128/MCB.00516-07. Epub 2007 Aug 20. Mol Cell Biol. 2007. PMID: 17709390 Free PMC article.
-
Disruption of the 5' stem-loop of yeast U6 RNA induces trimethylguanosine capping of this RNA polymerase III transcript in vivo.RNA. 2000 Dec;6(12):1859-69. doi: 10.1017/s1355838200991325. RNA. 2000. PMID: 11142384 Free PMC article.
References
Publication types
MeSH terms
Substances
Grants and funding
LinkOut - more resources
Full Text Sources
Other Literature Sources
Miscellaneous