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. 2013 Jan-Feb;21(1):74-9.
doi: 10.1590/1678-7757201302322.

Ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine

Esther Navarro-Escobar  1 , Pilar Baca, María Paloma González-Rodríguez, María Teresa Arias-Moliz, Matilde Ruiz, Carmen María Ferrer-Luque
Affiliations

Ex vivo microbial leakage after using different final irrigation regimens with chlorhexidine

Esther Navarro-Escobar et al. J Appl Oral Sci. 2013 Jan-Feb.

Abstract

Objective: To assess the influence of final irrigation protocols with chlorhexidine in the coronal leakage of Enterococcus faecalis in filled root canals.

Material and methods: Seventy single-root canals from extracted teeth were prepared using ProTaper instruments. The irrigation protocol accomplished an alternating irrigation with 5 mL of 2.5% sodium hypochlorite (NaOCI) and 17% EDTA between each file. The teeth were randomly divided into four experimental groups (n=15) according to the final irrigation regimen: group 1, without final irrigation; group 2, irrigation with 10 mL 2.0% chlorhexidine (CHX); group 3, with a final application of EC40™; and group 4, irrigation with the combination (1:1) of 0.2% CHX + 0.1% cetrimide (CTR). All the teeth were mounted in a two-chamber apparatus and the coronal access was exposed to E. faecalis. The presence of turbidity in the BHI broth over a period of 180 days was observed. The Friedman test was used for statistical analysis.

Results: EC40™ varnish showed the least leakage at 180 days, and was statistically similar to 2% CHX. No significant differences were observed between the group without final irrigation and the 2% CHX group or 0.2% CHX + 0.1% CTR.

Conclusions: In this ex vivo study, EC40™ showed the longest delayed coronal leakage of E. faecalis, although without significant differences from 2% CHX.

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Figures

Figure 1
Figure 1
Scanning electron microscopy (SEM) micrograph. E. faecalis biofilm attached to the root canal wall and entering the dentinal tubule
See this image and copyright information in PMC

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