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. 2011 Jan;1(1):20-2.
doi: 10.1016/S2221-1691(11)60062-2.

A transmission electron microscopy study of the diversity of Candida albicans cells induced by Euphorbia hirta L. leaf extract in vitro

Abu Arra Basma  1 , Zakaria Zuraini, Sreenivasan Sasidharan
Affiliations

A transmission electron microscopy study of the diversity of Candida albicans cells induced by Euphorbia hirta L. leaf extract in vitro

Abu Arra Basma et al. Asian Pac J Trop Biomed. 2011 Jan.

Abstract

Objective: To determine the major changes in the microstructure of Candida albicans (C. albicans) after treatment with Euphorbia hirta (E. hirta) L. leaf extract.

Methods: Transmission electron microscopy was used to study the ultrastructural changes caused by E. hirta extract on C. albicans cells at various exposure time.

Results: It was found that the main abnormalities were the alterations in morphology, lysis and complete collapse of the yeast cells after 36 h of exposure to the extract. Whereas the control cultures showed a typical morphology of Candida with a uniform central density, typically structured nucleus, and a cytoplasm with several elements of endomembrane system and enveloped by a regular, intact cell wall.

Conclusions: The significant antifungal activity shown by this methanol extract of E. hirta L. suggests its potential against infections caused by C. albicans. The extract may be developed as an anticandidal agent.

Keywords: Anticandidal agent; Candida albicans; Euphorbia hirta L.; Transmission electron microscopy.

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Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

Figures

Figure 1.
Figure 1.. TEM images of the untreated cells of C. albicans (after 24 h).
A: 4 000×; B: 5 000×; C: 10 000×; D: 16 000×.
Figure 2.
Figure 2.. TEM images of C. albicans cells after 12 h treatment with E. hirta L. leaf extract.
A: 4 000 ×; B: 5 000 ×; C: 10 000 ×; D: 16 000 ×.
Figure 3.
Figure 3.. TEM images of C. albicans cells after 24 h treatment with E. hirta L. leaf extract.
A: 4 000 ×; B: 5 000 ×; C: 10 000 ×; D: 16 000 ×.
Figure 4.
Figure 4.. TEM images of C. albicans cells after 36 h treatment with E. hirta L. leaf extract.
A: 4 000 ×; B: 5 000 ×; C: 10 000 ×; D: 16 000 ×.
See this image and copyright information in PMC

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