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. 2011 Aug;1(4):316-22.
doi: 10.1016/S2221-1691(11)60051-8.

Antidiabetic and in vitro antioxidant potential of Hybanthus enneaspermus (Linn) F. Muell in streptozotocin-induced diabetic rats

D K Patel  1 , R Kumar, S K Prasad, K Sairam, S Hemalatha
Affiliations

Antidiabetic and in vitro antioxidant potential of Hybanthus enneaspermus (Linn) F. Muell in streptozotocin-induced diabetic rats

D K Patel et al. Asian Pac J Trop Biomed. 2011 Aug.

Abstract

Objective: To evaluate antidiabetic and antioxidant potential of Hybanthus enneaspermus in different models.

Methods: The oral glucose tolerance test (OGTT) and normoglycemic effect of alcoholic extract of Hybanthus enneaspermus (AHE) were evaluated at a dose of 125, 250 and 500 mg/kg p.o. while hypoglycemic activity and effect on body weight were tested at 250 and 500 mg/kg p.o. per day for 21 days in Streptozotocin (STZ) induced diabetic rats. Further, glucose uptake by hemidiaphram was also evaluated. The total polyphenolic and flavonoid were determined and their correlation with various antioxidant assays was also determined.

Results: The results showed high level of phenolic content in AHE. AHE also exhibited higher total antioxidant capacity, good reducing power and a significant scavenger of reactive oxygen species like DPPH radical, nitric oxide, hydrogen peroxide and deoxyribose. Furthermore there was a significant increase in the body weight and decrease in the blood glucose level on treatment with the AHE. AHE increased glucose uptake on isolated rat hemi-diaphragm compared to control group.

Conclusions: AHE reduce blood glucose level in STZ-induced diabetic model. It does not show significant effect in normoglycemic study but showes significant effect in OGT. AHE has significant antioxidant activity, which may be attributed to high phenolic content.

Keywords: Antioxidant; Diabetic; Flavonoid; Flavonol; Glucose uptake; Hybanthus enneaspermus; Total phenol.

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Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

Figures

Figure 1.
Figure 1.. Anti-oxidant scavenging activity of AHE in DPPH scavenging assay.
Figure 2.
Figure 2.. Antioxidant scavenging activity of AHE in nitric oxid scavenging assay.
Figure 3.
Figure 3.. Anti-oxidant scavenging activity of AHE in H2O2 scavenging assay.
Figure 4.
Figure 4.. Anti-oxidant scavenging activity of AHE in hydroxyl radical scavenging assay.
Figure 5.
Figure 5.. Effect of AHE in OGTT.
*P<0.05, **P< 0.01, ***P<0.001, company with normal control.
Figure 6.
Figure 6.. Effect of AHE in normoglycemic study.
*P<0.05, **P<0.01, ***P<0.001, company with normal control.
See this image and copyright information in PMC

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