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. 2011 Dec;1(6):443-7.
doi: 10.1016/S2221-1691(11)60097-X.

Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia Limmonia Linn leaf

Mahendra Jain  1 , Rakhee Kapadia, Ravirajsinh N Jadeja, Menaka C Thounaojam, Ranjitsinh V Devkar, S H Mishra
Affiliations

Cytotoxicity evaluation and hepatoprotective potential of bioassay guided fractions from Feronia Limmonia Linn leaf

Mahendra Jain et al. Asian Pac J Trop Biomed. 2011 Dec.

Abstract

Objective: To evaluate the cytotoxicity and hepatoprotective potentials of extracts, fractions or isolated compound from the leaves of Feronia limonia (F. limonia).

Methods: Qualitative phytochemical analysis of extracts, fractions or compound was performed by means of thin layer chromatography and spectroscopic assays. The % purity of compound was measured by analytical HPLC. Extracts, fractions or compound have been individually evaluated for their cytotoxicity effects (10, 20, 100, 250, 500, 750 and 1 000 µg/mL). Based on the inhibitory concentration (IC50) obtained from the cell viability assay, graded concentrations of extracts, fractions or isolated compound were assessed (10, 20, 50, 100, 200 µg/mL) for its hepatoprotective potential against CCl4-induced hepatotoxicity by monitoring activity levels of serum glutamatic pyruvatic transaminase (SGPT) and serum glutamic oxaloacetic transaminase (SGOT).

Results: Results indicated that the methanol extract of F. limonia was non-toxic and hepatoprotective in nature as compared with the petroleum ether extract. The acetone fraction of methanolic extract also showed similar properties but the subsequent two fractions were cytotoxic. However, the pure compound isolated from the penultimate fraction of methanolic extract was non-toxic and hepatoprotective in nature. Biochemical investigations (SGOT, SGPT) further corroborated these cytological observations.

Conclusions: It can be concluded from this study that F. limonia methanol extract, some fractions and pure isolated compound herein exhibit hepatoprotective activity. However, cytotoxicity recorded in the penultimate fraction and investigation of structural details of pure compound warrants further study.

Keywords: Cytotoxicity; Feronia limonia leaf; HPLC; HepG2; Hepatoprotective; Hepatoprotective activity; Isolated compound; Phytochemical analysis; SGOT; SGPT; Spectroscopic assay; TLC.

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Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

Figures

Figure 1.
Figure 1.. TLC fingerprinting of extracts, fractions and isolated compound from F. limonia leaf at UV 365 nm.
Figure 2.
Figure 2.. Three-dimensional overlaid chromatogram of extracts, fractions and isolated compound from F. limonia leaf.
Figure 3.
Figure 3.. Parameters and chromatogram of isolated compound MR-2 by HPLC process.
Figure 4.
Figure 4.. Cytotoxicity evaluation of F. limonia leaf extracts, fractions, isolated compound (MR-2).
Figure 5.
Figure 5.. Effect of F. limonia extracts, fractions, isolated compound (MR-2) and sylimarin on CCL4 induced hepatotoxicity.
SYL: Sylimarin.
See this image and copyright information in PMC

References

    1. Kohen R, Nyska A. Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and methods for their quantification. Toxicol Pathol. 2002;30:620–650. - PubMed
    1. Vitaglione P, Morisco F, Caporaso N, Fogliano V. Dietary antioxidant compounds and liver health. Crit Rev Food Sci Nutr. 2004;44:575–586. - PubMed
    1. Wongnawa M, Thaina P, Bumrungwong N, Nitiruangjarat A, Muso A, Prasartthong V. Effect of Phyllanthus amarus Schum & Thonn. and its protective mechanism on paracetomol hepatotoxicity in rats. Acta Hortic. 2005;680:195–201.
    1. Sureshkumar SV, Mishra SH. Hepatoprotective effect of extracts from Pergularia daemia Forsk. J Ethnopharmacol. 2006;107:164–168. - PubMed
    1. Canter PH, Thomas H, Ernst E. Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology. Trends Biotechnol. 2005;23:180–185. - PubMed

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