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. 2012 Feb;2(2):159-62.
doi: 10.1016/S2221-1691(11)60213-X.

Strategies for production of active eukaryotic proteins in bacterial expression system

Orawan Khow  1 , Sunutcha Suntrarachun
Affiliations

Strategies for production of active eukaryotic proteins in bacterial expression system

Orawan Khow et al. Asian Pac J Trop Biomed. 2012 Feb.

Abstract

Bacteria have long been the favorite expression system for recombinant protein production. However, the flaw of the system is that insoluble and inactive proteins are co-produced due to codon bias, protein folding, phosphorylation, glycosylation, mRNA stability and promoter strength. Factors are cited and the methods to convert to soluble and active proteins are described, for example a tight control of Escherichia coli milieu, refolding from inclusion body and through fusion technology.

Keywords: Escherichia coli; Expression; Recombinant protein.

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Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

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