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. 2012 Mar;2(3):176-80.
doi: 10.1016/S2221-1691(12)60037-9.

Antioxidant and antibacterial activity of different parts of Leucas aspera

Ai Lan Chew  1 , Jeyanthi James Antony Jessica, Sreenivasan Sasidharan
Affiliations

Antioxidant and antibacterial activity of different parts of Leucas aspera

Ai Lan Chew et al. Asian Pac J Trop Biomed. 2012 Mar.

Abstract

Objective: To evaluate antioxidant, antimicrobial and cytotoxic activity of different parts (root, flower, leaf and stem) of Leucas aspera (L. aspera) (Labiatae).

Methods: Different parts of L. aspera were extracted with 80% (v/v) methanol. The methanol extracts were subjected to antioxidant, antimicrobial and brine shrimp lethality assay.

Results: All the extracts showed moderate to potent antioxidant activity, among which the root extract demonstrated the strongest antioxidant activity with the IC50 value of 6.552 µg/mL. Methanol extract of root possessed antioxidant activity near the range of vitamin E and thus could be a potential rich source of natural antioxidant. In case of antimicrobial screening, crude extracts of root, flower, leaf and stem showed notable antibacterial activity against tested microorganisms. The root extract showed the highest mean zone of inhibition ranging from 9.0-11.0 mm against tested microorganisms, at a concentration of 100 mg/mL. In the brine shrimp lethality bioassay, it was evident that the methanol root extract did not show significant toxicity. The LC50 value for 12 h and 24 h observation was 2.890 mg/mL and 1.417 mg/mL, respectively.

Conclusions: The present finding suggests that the methanol root extract of L. aspera could be developed as pharmaceutical products.

Keywords: Antimicrobial activity; Antioxidant activity; Cytotoxic activity; Inhibition of zone; Labiatae; Leucas aspera; Natural antioxidant.

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Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

Figures

Figure 1.
Figure 1.. Scavenging effect (%) of plant extracts and known antioxidants at 2.0 mg/mL.
a, b, c, d, e and f indicate that the mean percentage scavenging between the tested populations is significantly different.
Figure 2.
Figure 2.. Dose inhibition curve of total antioxidant activity of root extract.
Figure 3.
Figure 3.. Toxicity of crude extract of L. aspera root against brine shrimp larvae (Artemis salina)-12 h observation.
Figure 4.
Figure 4.. Toxicity of crude extract of L. aspera root against brine shrimp larvae (Artemia salina)-24 h observation.
See this image and copyright information in PMC

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