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. 2012 May;2(5):385-8.
doi: 10.1016/S2221-1691(12)60061-6.

In vivo antioxidant and hepatoprotective activity of methanolic extracts of Daucus carota seeds in experimental animals

Kamlesh Singh  1 , Nisha Singh, Anish Chandy, Ashish Manigauha
Affiliations

In vivo antioxidant and hepatoprotective activity of methanolic extracts of Daucus carota seeds in experimental animals

Kamlesh Singh et al. Asian Pac J Trop Biomed. 2012 May.

Abstract

Objective: To assess the In vivo antioxidFant and hepatoprotective activity of methanolic extract of Daucus carota (D. carota) seeds in experimental animals.

Methods: Methanolic extracts of D. carota seeds is used for hepatoprotection assessment. Oxidative stress were induced in rats by thioacetamide 100 mg/kg s.c, in four groups of rats (two test, standard and toxic control). Two test groups received D. carota seeds extract (DCSE) at doses of 200 mg/kg and 400 mg/kg. Standard group received silymarin (25 mg/kg) and toxic control received only thioacetamide. Control group received only vehicle. On the 8th day animals were sacrificed and liver enzyme like serum glutamic pyruvic transaminase (SGPT), serum glutamic-oxaloacetic transaminase (SGOT) and alkaline phosphatase (ALP) were estimated in blood serum and antioxidant enzyme like superoxide dismutase (SOD), catalase (CAT), glutathione reductase (GRD), glutathione peroxidase (GPX), glutathione-S-transferase (GST) and lipid peroxidation (LPO) were estimated in liver homogenate.

Results: A significant decrease in SGPT, SGOT and ALP levels was observed in all drug treated groups as compared to thioacetamide group (P < 0.001) and in case of antioxidant enzyme a significant (P < 0.001) increase in SOD, CAT, GRD, GPX and GST was observed in all drug treated groups as compared with thioacetamide group. But in case of LPO a significant (P < 0.001) reduction was observed as compared to toxic control group.

Conclusions: DCSE has contributed to the reduction of oxidative stress and the protection of liver in experimental rats.

Keywords: Antioxidant; Biochemical parameters; Daucus carota seeds extract; Hepatoprotection; Silymarin; Thioacetamide.

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Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

Figures

Figure 1.
Figure 1.. Histopathology of control group-treated with carboxy methylated cellulose only (40×).
Figure 2.
Figure 2.. Histopathology of toxic control group-treated with thioacetamide (40×).
Figure 3.
Figure 3.. Histopathology of Standrad: treated with Thioacetamide along with Silymarin (40×).
Figure 4.
Figure 4.. Histopathology of DCSE (200 mg/kg) treated group-treated with thioacetamide along with DCSE (40×).
Figure 5.
Figure 5.. Histopathology of DCSE (400 mg/kg) treated group-treated with thioacetamide along with DCSE (40×).
See this image and copyright information in PMC

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