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. 2012 Jun;2(6):469-73.
doi: 10.1016/S2221-1691(12)60078-1.

Antimicrobial potential of Actinomycetes species isolated from marine environment

S Valli  1 , Sugasini S Suvathi, O S Aysha, P Nirmala, Kumar P Vinoth, A Reena
Affiliations

Antimicrobial potential of Actinomycetes species isolated from marine environment

S Valli et al. Asian Pac J Trop Biomed. 2012 Jun.

Abstract

Objective: To evaluate the antimicrobial activity of Actinomycetes species isolated from marine environment.

Methods: Twenty one strains of Actinomycetes were isolated from samples of Royapuram, Muttukadu, Mahabalipuram sea shores and Adyar estuary. Preliminary screening was done using cross-streak method against two gram-positive and eight gram-negative bacteria. The most potent strains C11 and C12 were selected from which antibacterial substances were extracted. The antibacterial activities of the extracts were performed using Kirby-Bauer disc diffusion method. Molecular identification of those isolates was done.

Results: All those twenty one isolates were active against at least one of the test organisms. Morphological characters were recorded. C11 showed activity against Staphylococcus species (13.0±0.5 mm), Vibrio harveyi (11.0±0.2 mm), Pseudomonas species (12.0±0.3 mm). C12 showed activity against Staphylococcus species (16.0±0.4 mm), Bacillus subtilis (11.0±0.2 mm), Vibrio harveyi (9.0±0.1 mm), Pseudomonas species (10.0±0.2 mm). 16S rRNA pattern strongly suggested that C11 and C12 strains were Streptomyces species.

Conclusions: The results of the present investigation reveal that the marine Actinomycetes from coastal environment are the potent source of novel antibiotics. Isolation, characterization and study of Actinomycetes can be useful in discovery of novel species of Actinomycetes.

Keywords: 16S rRNA; Antibacterial substance; Antimicrobial activity; Cross-streak method; Kirby-Bauer disc diffusion method; Marine Actinomycetes; Molecular identification; Morphological character; Streptomyces.

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Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

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