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. 2013 Jan;3(1):1-7.
doi: 10.1016/S2221-1691(13)60015-5.

Anti-bacterial activity and brine shrimp lethality bioassay of methanolic extracts of fourteen different edible vegetables from Bangladesh

M Obayed Ullah  1 , Mahmuda Haque, Kaniz Fatima Urmi, Abu Hasanat Md Zulfiker, Elichea Synthi Anita, Momtaj Begum, Kaiser Hamid
Affiliations

Anti-bacterial activity and brine shrimp lethality bioassay of methanolic extracts of fourteen different edible vegetables from Bangladesh

M Obayed Ullah et al. Asian Pac J Trop Biomed. 2013 Jan.

Abstract

Objective: To investigate the antibacterial and cytotoxic activity of fourteen different edible vegetables methanolic extract from Bangladesh.

Methods: The antibacterial activity was evaluated using disc diffusion assay method against 12 bacteria (both gram positive and gram negative). The plant extracts were also screened for cytotoxic activity using the brine shrimp lethality bioassay method and the lethal concentrations (LC50) were determined at 95% confidence intervals by analyzing the data on a computer loaded with "Finney Programme".

Results: All the vegetable extracts showed low to elevated levels of antibacterial activity against most of the tested strains (zone of inhibition=5-28 mm). The most active extract against all bacterial strains was from Xanthium indicum which showed remarkable antibacterial activity having the diameter of growth inhibition zone ranging from 12 to 28 mm followed by Alternanthera sessilis (zone of inhibition=6-21 mm). All extracts exhibited considerable general toxicity towards brine shrimps. The LC50 value of the tested extracts was within the range of 8.447 to 60.323 µg/mL with respect to the positive control (vincristine sulphate) which was 0.91 µg/mL. Among all studied extracts, Xanthium indicum displayed the highest cytotoxic effect with LC50 value of 8.447 µg/mL.

Conclusions: The results of the present investigation suggest that most of the studied plants are potentially good source of antibacterial and anticancer agents.

Keywords: Antibacterial; Bangladeshi vegetables; Brine shrimp lethality; Disc diffusion.

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Conflict of interest statement

Conflict of interest statement: We declare that we have no conflict of interest.

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