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. 2008 Jul;14(3):167-72.
doi: 10.1007/s12298-008-0015-1. Epub 2008 Sep 27.

Partial purification of superoxide dismutase and peroxidase from ber (Zizyphus mauritiana Lamk.) fruit using anion exchange chromatography

Sunil Kumar  1 , Sarla P Malhotra
Affiliations

Partial purification of superoxide dismutase and peroxidase from ber (Zizyphus mauritiana Lamk.) fruit using anion exchange chromatography

Sunil Kumar et al. Physiol Mol Biol Plants. 2008 Jul.

Abstract

Partial purification of the two enzymes i.e. superoxide dismutase (SOD) and peroxidase (POX) from ber pulp has been obtained by passing the ammonium sulphate fraction through a diethyl amino ethyl-cellulose (DEAE-Cellulose) column. The fractions showing SOD and POX activity were pooled separately and passed through a Sephadex G100 column for further purification. SOD was purified 12.2 fold with 12.6% yield while POX was purified 15.6 fold with 19.3% yield. Approximate molecular mass for SOD and POX, as judged by gel filtration method was 35.6 and 81.5 kDa, respectively.

Keywords: Ber fruit; DEAE-cellulose chromatography; Zizyphus mauritiana Lamk; fold purification; peroxidase; superoxide dismutase; yield.

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