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. 2009 Jul;15(3):257-65.
doi: 10.1007/s12298-009-0029-3. Epub 2009 Oct 28.

Impact of cefotaxime on somatic embryogenesis and shoot regeneration in sugarcane

Pallavi Mittal  1 , Satbir Singh Gosal, Anuj Senger, Pradeep Kumar
Affiliations

Impact of cefotaxime on somatic embryogenesis and shoot regeneration in sugarcane

Pallavi Mittal et al. Physiol Mol Biol Plants. 2009 Jul.

Abstract

A cephalosporin antibiotic, cefotaxime (Omnatax™) promoted somatic embryogenesis and subsequent shoot regeneration in vitro from spindle in sugarcane irrespective of the genotypes as (CoJ 83, CoJ 88 and CoJ 64) culturered on MS medium with 2,4-D (2.5 mgl(-1)) and kinetin (0.5 mgl(-1)). Seven different concentrations of cefotaxime (100, 200, 300, 400, 500, 600 and 700 mgl(-1)) were tested to find the optimal concentration of cefotaxime for somatic embryogenesis from callus cultures. Among the three varieties, calli of variety CoJ 83 incubated on MS medium with 2,4-D (2.5 mgl(-1)) + kinetin (0.5 mgl(-1)) + cefotaxime (500 mgl(-1)) exhibited maximum somatic embryogenesis. To improve shoot regeneration, the callus was transferred to MS medium with BAP (0.5 mgl(-1)) + kinetin (0.5 mgl(-1)) in combination with different levels of cefotaxime. Highest frequency of shoot regeneration was observed in callus of CoJ 83 in the presence of 500 mgl(-1) cefotaxime. The plantlets could be successfully hardened in polybags and transferred to soil, where they exhibited normal growth. Our results convincingly demonstrated that cefotaxime improves somatic embryogenesis from spindle and regeneration from embryogenic calli of sugarcane and hence can be strongly recommended for rapid and large scale multiplication of sugarcane.

Keywords: Saccharum officinarum L.; antibiotic; callus; leaf segments; plant regeneration.

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