Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Winter;14(4):254-63.
Epub 2013 Feb 20.

Chromosome abnormalities and viability of vitrified eight-cell mouse embryos at presence of two different cryoprotectants at different storage durations

Shabnam Zarei Moradi  1 , Anahita Mohseni Meybodi, Hamid Gourabi, Hossein Mozdarani, Zahra Mansouri
Affiliations

Chromosome abnormalities and viability of vitrified eight-cell mouse embryos at presence of two different cryoprotectants at different storage durations

Shabnam Zarei Moradi et al. Cell J. 2013 Winter.

Abstract

Objective: Experiments were conducted to find the differences between post-thaw viability and chromosome aberrations in eight-cell mouse embryos at presence of dimethyl sulfoxide (DMSO) and 1, 2-propanediol (PROH) as croprotectants in different storage durations.

Materials and methods: In this case-control study, a total number of 720 mouse embryos from about 250 NMRI mice were vitrified with 30% PROH or DMSO; each diluted with a solution containing 30% ficol plus 0.5 M sucrose. Embryos were exposed to the solutions for 0.5 minute at 25℃ followed by cooling in liquid nitrogen, then after appropriate storage duration, they were rapidly warmed. Besides, there were 100 mouse embryos for each cryoprotectant group (totally 200 embryos) as control. Embryo survival was assessed by in vitro development, and chromosome abnormalities were analyzed by Giemsa staining.

Results: The proportion of mitotic abnormalities in PROH/DMSO vitrified embryos was significantly higher than unfrozen control group. This was confirmed also by a reduced viability of the embryos as judged by a culture at the blastocyst stage (p<0.05 in all test groups).

Conclusion: It can be deduced that long term cryopreservation may result in chromosomal abnormalities and/or low viability.

Keywords: Chromosome Abnormality; Cryopreservation; Mouse Embryo; Viability.

PubMed Disclaimer

Figures

Fig 1
Fig 1
Percentage of viability at the presence of DMSO and PROH as cryoprotectant for various storage durations . Error bars show SE of mean values calculated for data obtained from different samples.
Fig 2
Fig 2
Percentage of chromosome abnormality at the presence of DMSO and PROH as cryoprotectant for various storage durations. Error bars show SE of mean values calculated for data obtained from different samples.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Trounson A. Preservation of human eggs and embryos. Fertil Steril. 1986;46(1):1–12. - PubMed
    1. Bergh C, Werner C, Nilsson L, Hamberger L. Cumulative birth rates following cryopreservation of all embryos in stimulated in vitro fertilization (IVF) cycles. J Assist Reprod Genet. 1995;12(3):191–194. - PubMed
    1. Ombelet W, De Sutter P, Van der Elst J, Martens G. Multiple gestation and infertility treatment: registration, reflection and reaction-the Belgian project. Hum Reprod Update. 2005;11(1):3–14. - PubMed
    1. Van Landuyt L, Verheyen G, Tournaye H, Camus M, Devroey P, Van Steirteghem A. New Belgian embryo transfer policy leads to sharp decrease in multiple pregnancy rate. Reprod Biomed Online. 2006;13(6):765–771. - PubMed
    1. Neubourg DD, Mangelschots K, Van Royen E, Vercruyssen M, Ryckaert G, Valkenburg M, et al. Impact of patients' choice for single embryo transfer of a top quality embryo versus double embryo transfer in the first IVF/ICSI cycle. Hum Reprod. 2002;17(10):2621–2625. - PubMed

LinkOut - more resources