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. 2013 Jun;79(12):3892-6.
doi: 10.1128/AEM.00182-13. Epub 2013 Apr 12.

Identification of potentially diarrheagenic atypical enteropathogenic Escherichia coli strains present in Canadian food animals at slaughter and in retail meats

Raven Comery  1 , Ajitha Thanabalasuriar, Philippe Garneau, Andrea Portt, Patrick Boerlin, Richard J Reid-Smith, Josée Harel, Amee R Manges, Samantha Gruenheid
Affiliations

Identification of potentially diarrheagenic atypical enteropathogenic Escherichia coli strains present in Canadian food animals at slaughter and in retail meats

Raven Comery et al. Appl Environ Microbiol. 2013 Jun.

Abstract

This study identified and characterized enteropathogenic Escherichia coli (EPEC) in the Canadian food supply. Eighteen of 450 E. coli isolates from food animal sources were identified as atypical EPEC (aEPEC). Several of the aEPEC isolates identified in this study possessed multiple virulence genes, exhibited adherence and attaching and effacing (A/E) lesion formation, disrupted tight junctions, and were coclassified with the extraintestinal pathogenic E. coli (ExPEC) and enterotoxigenic E. coli (ETEC) pathotypes.

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Figures

Fig 1
Fig 1
Clustering analysis of 18 aEPEC strains and reference strains based on virulence gene profiles. Distance between isolate profiles was done with the MeV software suite. Distance between isolate profiles was calculated using a single-linkage Pearson square formula with 1,000 bootstrap iterations. Bootstrap values for each node are indicated in boxes.
Fig 2
Fig 2
Sample Western blot showing secretion of Tir and NleA. Lab strain E2348//69 wild-type tEPEC was used as a positive control, and a ΔescN EPEC strain was used as a negative control.
Fig 3
Fig 3
Immunofluorescence microscopy images showing pedestal formation caused by aEPEC strains. Strain AB05-0342 shows actin recruitment at sites of bacterial attachment but very few pedestals.
Fig 4
Fig 4
Immunofluorescence microscopy images showing tight junction disruption caused by three aEPEC strains.
See this image and copyright information in PMC

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