Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Jun;30(5):703-10.
doi: 10.1007/s10815-013-9992-2. Epub 2013 Apr 13.

The use of morphokinetic parameters to select all embryos with full capacity to implant

Sandrine Chamayou  1 , Pasquale Patrizio, Giorgia Storaci, Venera Tomaselli, Carmelita Alecci, Carmen Ragolia, Claudia Crescenzo, Antonino Guglielmino
Affiliations

The use of morphokinetic parameters to select all embryos with full capacity to implant

Sandrine Chamayou et al. J Assist Reprod Genet. 2013 Jun.

Abstract

Purpose: Embryo kinetics analysis is an emerging tool for selecting embryo(s) for transfer. The aim of the present study was to determine morphokinetic parameters easily usable in the laboratory and predictive of embryo development and, most importantly, of embryo competence in producing a clinical pregnancy after day 5 transfer.

Methods: A retrospective time-lapse monitoring analysis of morphokinetic parameters for 72 fully implanted embryos (group A) were compared to 106 non-implanted embryos (group B), and to 66 embryos with arrested development from the same pool of group A. All the embryos were from 78 patients undergoing ICSI treatment and day 5 embryo transfers.

Results: A day 3 embryo will develop into a viable blastocyst if the following ranges of morphokinetic parameters are met: t1 (between 18.4 h and 30.9 h post-ICSI), t2 (21.4-34.8 h), t4 (33.1-57.2 h), t7 (46.1-82.5 h), t8 (46.4-97.8 h), tC-tF (7.7-22.9 h) and s3 (0.7-30.8 h). On day 5 embryos with the highest probability to implant are those with a cc3 between 9.7 h and 21 h.

Conclusions: Morphokinetic parameters are helpful to make appropriate decisions for the disposition of each embryo. It is recommended that each laboratory should determine its own ranges of in vitro development (IVD-MKP) and implantation-associated (IMP-MKP) morphokinetic parameters.

PubMed Disclaimer

Figures

Fig. 1
Fig. 1
Morphokinetic parameters from t0 to tEB (2PN: two pronuclei; 1C: 1 cell-stage,…, 9+: 9 cell-stage or more separated cells by individual membranes, M:morula, B:Blastocyst, EB: expanded blastocyst)
Fig. 2
Fig. 2
Embryonic kinetic parameters determining the capacity of an embryo to become a viable embryo on the fifth day of in vitro culture (group A-Imp: green) compared with embryos with impaired development (group A-NU : red). The kinetic parameters are expressed in hour and fraction of an hour
Fig. 3
Fig. 3
CC3 value of competent day 5 embryo to implant (Group A-Imp: Green) and embryos that fail to implant if transferred on day 5 (Group B: Orange). The kinetic parameters are expressed in hour and fraction of an hour
See this image and copyright information in PMC

References

    1. Alpha Scientists in Reproductive Medicine and ESHRE Special Interest Group of Embryology The Istanbul consensus workshop on embryo assessment: proceedings of an expert meeting. Hum Reprod. 2011;26(6):1270–83. doi: 10.1093/humrep/der037. - DOI - PubMed
    1. Arav A, Aroyo A, Yavin S, Roth Z. Prediction of embryonic developmental competence by time-lapse observation and ‘shortest-half’ analysis. Reproductive BioMedicine on line. 2008;17(5):669–75. doi: 10.1016/S1472-6483(10)60314-8. - DOI - PubMed
    1. Azzarello A, Hoest T, Mikkelsen AL. The impact of pronuclei morphology and dynamicity on live birth outcome after time-lapse culture. Hum Reprod. 2012;27(9):2649–57. doi: 10.1093/humrep/des210. - DOI - PubMed
    1. Ciray HN, Aksay T, Goktas C, Ozturk B, Bahceci M. Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study. J Assist Reprod Genet. 2012;29(9):891–900. doi: 10.1007/s10815-012-9818-7. - DOI - PMC - PubMed
    1. Cruz M, Gadea B, Garrido N, et al. Embryo quality, blastocyst and ongoing pregnancy rates in oocyte donation patients whose embryos were monitored by time-lapse imaging. J Assist Reprod Genet. 2011;28(7):569–73. doi: 10.1007/s10815-011-9549-1. - DOI - PMC - PubMed