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. 2012 Winter;17(4):268-72.

Myocardial infarct size and area at risk assessment in mice

Bjorn Redfors  1 , Yangzhen Shao, Elmir Omerovic
Affiliations

Myocardial infarct size and area at risk assessment in mice

Bjorn Redfors et al. Exp Clin Cardiol. 2012 Winter.

Abstract

Mouse models of myocardial ischemia and infarction are important in cardiovascular research. Reliable and reproducible assessment of the area at risk (AAR) and infarct size (IS) in mice is vital for deciphering mechanisms behind these common diseases, and for developing and evaluating treatment strategies. The present review will briefly describe and discuss the most common methods for determining the AAR and IS in mouse models of cardiovascular disease. Several methods exist for ex vivo assessment of IS. Conventional histological stains target the fibrous scar and require several days to pass from the time of infarct induction until the animal is euthanized, whereas triphenyltetrazolium-based techniques stain the viable tissue surrounding the infarct and can be performed on tissue harvested within a few hours after infarction. The AAR is usually stained by injecting a dye into the circulation. This dye subsequently distributes to perfused tissue but leaves the AAR unstained. In vivo assessment enables serial measurements of the IS and/or AAR and is sometimes preferable to ex vivo techniques. Echocardiography is usually the method of choice but magnetic resonance imaging-based techniques are also used. The aim of the present review was to provide basic researchers with an introduction to the various techniques used to assess and quantify the IS and AAR in experimental mouse models of myocardial ischemia-reperfusion and infarction.

Keywords: Area at risk; Echocardiography; Infarct size; Myocardial infarction.

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Figures

Figure 1)
Figure 1)
2,3,5-Triphenyltetrazolium staining after an experimentally induced myocardial infarction. Noninfarcted, viable tissue is stained red, whereas the infarcted region is left unstained. The image was provided by Dr J Downey, University of South Alabama (Mobile, Alabama, USA)
Figure 2)
Figure 2)
Area at risk (AAR) assessment using Evans Blue dye. For visualization of the AAR, Evans Blue dye is injected into the circulation and distributes to the perfused parts of the myocardium. Nonperfused areas, ie, the AAR, remain unstained
See this image and copyright information in PMC

References

    1. Ytrehus K. The ischemic heart: Experimental models. Pharmacol Res. 2000;42:193–203. - PubMed
    1. Doevendans PA, Daemen MJ, de Muinck ED, Smits JF. Cardiovascular phenotyping in mice. Cardiovasc Res. 1998;39:34–49. - PubMed
    1. Liu Z, Kastis GA, Stevenson GD, et al. Quantitative analysis of acute myocardial infarct in rat hearts with ischemia-reperfusion using a high-resolution stationary SPECT system. J Nucl Med. 2002;43:933–9. - PMC - PubMed
    1. Califf RM, Phillips HR, III, Hindman MC, et al. Prognostic value of a coronary artery jeopardy score. J Am Coll Cardiol. 1985;5:1055–63. - PubMed
    1. Graham MM, Faris PD, Ghali WA, et al. Validation of three myocardial jeopardy scores in a population-based cardiac catheterization cohort. Am Heart J. 2001;142:254–61. - PubMed