Matrix-assisted laser desorption ionization-time of flight mass spectrometry in clinical microbiology

Abstract

Despite widespread application of nucleic acid diagnostics, cultures remain integral in modern laboratories. Because cultures detect a large number of organism types, it is unlikely that they will disappear from clinical practice in the near future. Their downside is slow turn-around time, impacted by time to growth and identification of that growth. The latter is expedited using a new proteomic technology, matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS).

Keywords: MALDI-TOF; bacteria; fungi; mass spectrometry; matrix-assisted laser desorption ionization-time of flight.