Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2013 Apr;5(4):1231-1235.
doi: 10.3892/etm.2013.952. Epub 2013 Feb 6.

Study of the biological effectiveness of a nanosilver-epidermal growth factor sustained-release carrier

Jian-DA Zhou  1 , Shao-Hua Wang, Rui Liu, Chun-Jiao Zhou, Ke Cao, Jin-Yan Liu, Yao Chen, Feng-Hua Chen
Affiliations

Study of the biological effectiveness of a nanosilver-epidermal growth factor sustained-release carrier

Jian-DA Zhou et al. Exp Ther Med. 2013 Apr.

Abstract

The aim of the present study was to elucidate the biological effectiveness and character of a nanosilver-epidermal growth factor (EGF) sustained-release carrier. This was synthesized using the self-assembly method and then characterized by transmission electron microscopy and UV spectrophotometry. The biological activity of the sustained release carrier was determined through cytological, bacteriological and wound-healing experiments. The results showed that the nanosilver-EGF sustained-release carrier was well dispersed with uniform particle size and that it had good antibacterial properties similar to those of nanosilver. The nanosilver-EGF sustained-release carrier is superior to EGFs in effectively promoting cell division and proliferation. The results of the wound-healing experiments provide evidence of its curative effects.

Keywords: antibacterial; cell proliferation; epidermal growth factor; nanosilver; wound.

PubMed Disclaimer

Figures

Figure 1
Figure 1
Transmission electron microscopic image of the silver nanoparticles (magnification, ×500,000).
Figure 2
Figure 2
Transmission electron microscopic image of the nanosilver-EGF complex (×500,000). EGF, epidermal growth factor.
Figure 3
Figure 3
Ultraviolet absorption spectrum of nanosilver-EGF complex. (1) Absorption spectrum of EGF. (2, 3) Absorption spectrum of nanosilver-alone group (100 and 500 ppm, respectively). (4) Absorption spectrum of nanosilver-EGF sustained-release group (25 ppm). EGF, epidermal growth factor.
Figure 4
Figure 4
Light microscopic image of human dermal fibroblasts at 36 h subsequent to hematoxylin-eosin staining in the nanosilver-EGF complex group. EGF, epidermal growth factor.
Figure 5
Figure 5
Proliferation of human dermal fibroblasts in each group at 12, 24, 36 and 48 h. EGF, epidermal growth factor; NanoAg-EGF, nanosilver-epidermal growth factor sustained-release carrier; NanoAg+EGF, nanosilver-EGF combination; NanoAg, nanosilver-alone.
Figure 6
Figure 6
Inhibitory action of the experimental group against five pathogenic microorganisms. (1) NanoAg-EGF group. (2) NanoAg group. (3) Benzylpenicillin positive control group. (4) EGF group. (5) Normal control group. EGF, epidermal growth factor; NanoAg-EGF, nanosilver-epidermal growth factor sustained-release carrier; NanoAg, nanosilver-alone.
Figure 7
Figure 7
Comparison of the wound-healing rate in each group at 3, 7 and 12 days post-trauma. EGF, epidermal growth factor; NanoAg-EGF, nanosilver-epidermal growth factor sustained-release carrier; NanoAg+EGF, nanosilver-EGF combination; NanoAg, nanosilver-alone.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ, Kim SH, Park YK, Park YH, Hwang CY, Kim YK, Lee YS, Jeong DH, Cho MH. Antimicrobial effects of silver nanoparticles. Nanomedicine. 2007;3:95–101. - PubMed
    1. Chekman IS, Ulberg ZR, Gorchakova NO, Nebesna TY, Gruzina TG, Priskoka AO, Doroshenko AM, Simonov PV. The prospects of medical application of metal-based nanoparticles and nanomaterials. Lik Sprava. 2011:3–21. - PubMed
    1. Dallas P, Sharma VK, Zboril R. Silver polymeric nanocomposites as advanced antimicrobial agents: classification, synthetic paths, applications, and perspectives. Adv Colloid Interface Sci. 2011;166:119–135. - PubMed
    1. Arora S, Jain J, Rajwade JM, Paknikar KM. Cellular responses induced by silver nanoparticles: In vitro studies. Toxicol Lett. 2008;179:93–100. - PubMed
    1. Ai J, Biazar E, Jafarpour M, Montazeri M, Majdi A, Aminifard S, Zafari M, Akbari HR, Rad HG. Nanotoxicology and nanoparticle safety in biomedical designs. Int J Nanomedicine. 2011;6:1117–1127. - PMC - PubMed

LinkOut - more resources