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. 2013 Feb 15:105:317-9.
doi: 10.1016/j.talanta.2012.10.045. Epub 2012 Nov 2.

Alkaloid chelirubine and DNA: blue and red luminescence

Michal Rajecky  1 , Iva Slaninova, Petra Mokrisova, Jana Urbanova, Martin Palkovsky, Eva Taborska, Petr Taborsky
Affiliations

Alkaloid chelirubine and DNA: blue and red luminescence

Michal Rajecky et al. Talanta. .

Abstract

Extracts from Himalayan herb Dicranostigma lactucoides containing alkaloid chelirubine have been used for centuries in Chinese herbal medicine. We have found a new utilization for the alkaloid: it can be used as a DNA fluorescent probe showing blue (free form) and red (intercalated to DNA) luminescence emission after irradiation by near-UV light. Besides quantification of DNA (LOD = 6 ng ml(-1)) it can also be used as a supravital cell probe because chelirubine molecules can effectively enter into the living cell through the cell membrane.

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