A poly(U) polymerase in tobacco leaves
- PMID: 235994
- DOI: 10.1016/0005-2787(75)90304-4
A poly(U) polymerase in tobacco leaves
Abstract
A poly(U) polymerizing enzyme has been found in healthy and tobacco mosaic virus-infected tobacco leaves and has been partially purified by affinity chromatography on a gel prepared from agarose with chemically coupled RNA. The enzyme is stimulated by Mn-2+ and dependent on a polynucleotide, preferentially poly(A). The synthesis proceeds optimally at pH 7.6 and 25 degrees C. The enzyme is highly specific for UTP and is inhibited by other ribonucleoside triphosphates. The product was partly sensitive to pancreatic ribonuclease. The synthetic reaction is inhibited in the presence of pyrophosphate but insensitive to 10 mM orthophosphate and high levels of cordycepin, rifampicin and actinomycin D. A molecular weight of about 40,000 has been estimated by sucrose gradient analysis and partition cell ultracentrifugation.
Similar articles
-
RNA-synthesizing enzymes in healthy and TMV-infected tobacco leaves. Partial purification and characterization to tobacco polynucleotide phosphorylase.Arch Biochem Biophys. 1974 Sep;164(1):224-32. doi: 10.1016/0003-9861(74)90026-5. Arch Biochem Biophys. 1974. PMID: 4429350 No abstract available.
-
RNA-synthesizing enzymes in healthy and TMV-infected tobacco leaves. Separation and properties of enzymes catalyzing nucleotide polymerization.Arch Biochem Biophys. 1974 Sep;164(1):218-23. doi: 10.1016/0003-9861(74)90025-3. Arch Biochem Biophys. 1974. PMID: 4429349 No abstract available.
-
Identification and characterization of an RNA replicase from TMV-infected tobacco leaves.Biochem Biophys Res Commun. 1970 Oct 23;41(2):506-11. doi: 10.1016/0006-291x(70)90535-8. Biochem Biophys Res Commun. 1970. PMID: 5518180 No abstract available.
-
Separation studies of TMV replicase.Ann Microbiol (Paris). 1976 Jan;127A(1):25-31. Ann Microbiol (Paris). 1976. PMID: 1275401
-
Replication of tobacco mosaic virus. II. The in vitro synthesis of high molecular weight virus-specific RNAs.Virology. 1971 Jul;45(1):192-9. doi: 10.1016/0042-6822(71)90126-7. Virology. 1971. PMID: 5112411 No abstract available.
Cited by
-
Purification of a terminal uridylyltransferase that acts as host factor in the in vitro poliovirus replicase reaction.Proc Natl Acad Sci U S A. 1986 Jan;83(2):221-5. doi: 10.1073/pnas.83.2.221. Proc Natl Acad Sci U S A. 1986. PMID: 2417240 Free PMC article.
-
RNA end-labeling and RNA ligase activities can produce a circular rRNA in whole cell extracts from trypanosomes.Nucleic Acids Res. 1987 Apr 24;15(8):3275-90. doi: 10.1093/nar/15.8.3275. Nucleic Acids Res. 1987. PMID: 2437529 Free PMC article.
-
Terminal uridylyl transferase of Vigna unguiculata: purification and characterization of an enzyme catalyzing the addition of a single UMP residue to the 3'-end of an RNA primer.Nucleic Acids Res. 1981 Jun 11;9(11):2433-53. doi: 10.1093/nar/9.11.2433. Nucleic Acids Res. 1981. PMID: 6269049 Free PMC article.
-
A novel gene expression pathway regulated by nuclear phosphoinositides.Adv Enzyme Regul. 2009;49(1):11-28. doi: 10.1016/j.advenzreg.2009.01.007. Adv Enzyme Regul. 2009. PMID: 19534024 Free PMC article. Review. No abstract available.
-
Comparison of endogenous and exogenous RNA primers of poly(U) polymerase in rat hepatic ribosomes.Biochem J. 1979 Mar 1;177(3):895-902. doi: 10.1042/bj1770895. Biochem J. 1979. PMID: 444210 Free PMC article.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
