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Observational Study
. 2013;18(3):100-6.
doi: 10.1179/1351000213Y.0000000045. Epub 2013 Apr 19.

Altered redox status in the blood of psoriatic patients: involvement of NADPH oxidase and role of anti-TNF-α therapy

V V Barygina  1 , M Becatti, G Soldi, F Prignano, T Lotti, P Nassi, D Wright, N Taddei, C Fiorillo
Affiliations
Observational Study

Altered redox status in the blood of psoriatic patients: involvement of NADPH oxidase and role of anti-TNF-α therapy

V V Barygina et al. Redox Rep. 2013.

Abstract

Background: Psoriasis is a chronic hyperproliferative inflammatory skin disease, characterized by a generalized redox imbalance. Anti-tumor necrosis factor (TNF)-α therapy is widely used for the treatment of this disease, but its effect on blood redox status hasn't been explored.

Objective: To investigate the effects of anti-TNF-α therapy on blood redox status in psoriatic patients.

Methods: Twenty-nine psoriatic patients (PSO) were divided into two groups: one remained untreated (NRT) and to another the anti-TNF-α therapy was prescribed (TR). The levels of main oxidative stress markers and total antioxidant capacity (TAC) in plasma, levels of total reactive oxygen species (ROS) production, lipoperoxidation, TAC, glutathione content, and activity of NADPH oxidase in white blood cells (WBC) were evaluated in PSO, in NTR and TR after 6 months of the study.

Results: Plasma levels of malondialdehyde (MDA) and protein carbonyl content (PCO), ROS production, lipoperoxidation, and glutathione content in WBC were increased, while TAC in both plasma and WBC was decreased in PSO with respect to controls. In the plasma of TR, levels of MDA and PCO were significantly lower with respect to PSO and NTR. The activity of NADPH oxidase was significantly increased in WBC of PSO and NTR but not in TR versus controls.

Discussion: Our results represent novel data about the redox status of WBC in psoriatic patients. A significant redox-balancing effect of anti-TNF-α therapy, probably associated with the normalization of NADPH oxidase activity in WBC, was demonstrated.

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Figures

Figure 1.
Figure 1.
NADPH oxidase activity in intact PBMC, measured by lucigenin-based assay. PSO, psoriatic patients prior to anti-TNF-α therapy (n = 29); NTR, psoriatic patients without any systematic treatment after 6 months (n = 16); TR; psoriatic patients after 6 months of anti-TNF-alpha therapy (n = 13). SOD, superoxide dismutase. *P < 0.05 as compared to control; #P < 0.05 as compared to NTR.
See this image and copyright information in PMC

References

    1. Coimbra S, Figueiredo A, Castro E, Rocha-Pereira P, Santos-Silva A. The roles of cells and cytokines in the pathogenesis of psoriasis. Int J Dermatol 2012;51(4):389–95. - PubMed
    1. Cai Y, Fleming C, Yan J. New insights of T cells in the pathogenesis of psoriasis. Cell Mol Immunol 2012;9(4):302–9. - PMC - PubMed
    1. Zhou Q, Mrowietz U, Rostami-Yazdi M. Oxidative stress in the pathogenesis of psoriasis. Free Radic Biol Med 2009;47:891–905. - PubMed
    1. Gabr SA, Al-Ghadir AH. Role of cellular oxidative stress and cytochrome c in the pathogenesis of psoriasis. Arch Dermatol Res 2012;304(6):451–7. - PubMed
    1. Pujari VM, Suryakar AN, Ireddy S. Oxidants and antioxidant status in psoriasis patients. Biomed Res 2010;21(2):221–3.

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