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. 1986 Mar;2(1):9-17.
doi: 10.1007/BF03191957.

Isolation of secondary metabolites of Aspergillus ochraceus by HPLC

I Delgadillo  1
Affiliations

Isolation of secondary metabolites of Aspergillus ochraceus by HPLC

I Delgadillo. Mycotoxin Res. 1986 Mar.

Abstract

A method is described for the isolation and purification of ochratoxin A, ochratoxin B, ochratoxin ß mellein, 4-hydroxymellein and penicillic acid produced byAspergillus ochraceus in a synthetic liquid medium. Ochratoxin α, which was not found in the culture medium, was obtained by acid hydrolysis of ochratoxin A. A high pressure liquid Chromatograph equipped with Lichrosorb 100 and Lichrosorb RP-18 columns and UV and/or Refractive Index detectors was used.

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