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. 1990 Sep;6(2):85-8.
doi: 10.1007/BF03192147.

Fumonisin B1: Isolation from corn culture, and purification by high performance liquid chromatography

R Vesonder  1 , R Peterson, R Plattner, D Weisleder
Affiliations

Fumonisin B1: Isolation from corn culture, and purification by high performance liquid chromatography

R Vesonder et al. Mycotoxin Res. 1990 Sep.

Abstract

A method is described to isolate fumonisin B1 (FB1) from corn cultured for 18 days at 25°C withFusarium moniliforme. Cultured corn was extracted with aqueous methanol and purified with XAD-2 column chromatography and high performance liquid chromatography (HPLC). About 450 mg of FB1 were obtained from 800g cultured corn. Its identity was established by fast-atom bombardment (FAB) mass spectrometry, and infrared spectrum and nuclear magnetic spectrum. Its purity was estimated to be 95% by gas chromatography/mass spectrometry (GC/MS).References.

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References

    1. Appl Environ Microbiol. 1988 Jul;54(7):1806-11 - PubMed
    1. Onderstepoort J Vet Res. 1988 Dec;55(4):197-203 - PubMed

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