Entwicklung eines immunchemischen Schnelltestverfahrens zum Multimykotoxinnachweis

Abstract

A rapid immunochemical test system (immunofiltration, flow-through test) was developed for the simultaneous detection of seven mycotoxins, namely aflatoxin B1 (AFB1), fumonisin B1 (FB1), ochratoxin A (OA), deoxynivalenol (DON), T-2 toxin (T-2), diacetoxyscirpenol (DAS), and roridin A (RoA), respectively. A reusable test device, containing seven sample wells and one control well was produced. Sample extract solutions were dropped onto each sample well and the controll well, followed by toxin-enzyme conjugate solution, wash solution and colour delevoper solution. The test results were evaluated by visual comparison of colour intensity of sample wells and control well. The detection limits in buffer solution were at 0.5 ng/ml (AFB1), 5 ng/ml (FB1), 5 ng/ml (OA), 500 ng/ml (DON), 10 ng/ml (T-2), 0.5 ng/ml (DAS), and 25 ng/ml (RoA), respectively. Employing a simple extraction procedure, artificially contaminated cereal samples (wheat, maize) were analysed, with detection limits of 10 ng/g (AFB1), 50 ng/g (FB1), 50 ng/g (OA), 3500 ng/g (DON), 100 ng/g (T-2), 5 ng/g (DAS), and 250 ng/g (RoA), respectively.