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. 2013:2013:681891.
doi: 10.1155/2013/681891. Epub 2013 Mar 27.

The antibacterial effect of ethanol extract of polish propolis on mutans streptococci and lactobacilli isolated from saliva

Arkadiusz Dziedzic  1 , Robert Kubina, Robert D Wojtyczka, Agata Kabała-Dzik, Marta Tanasiewicz, Tadeusz Morawiec
Affiliations

The antibacterial effect of ethanol extract of polish propolis on mutans streptococci and lactobacilli isolated from saliva

Arkadiusz Dziedzic et al. Evid Based Complement Alternat Med. 2013.

Abstract

Dental caries occurrence is caused by the colonization of oral microorganisms and accumulation of extracellular polysaccharides synthesized by Streptococcus mutans with the synergistic influence of Lactobacillus spp. bacteria. The aim of this study was to determine ex vivo the antibacterial properties of ethanol extract of propolis (EEP), collected in Poland, against the main cariogenic bacteria: salivary mutans streptococci and lactobacilli. The isolation of mutans streptococci group bacteria (MS) and Lactobacillus spp. (LB) from stimulated saliva was performed by in-office CRT bacteria dip slide test. The broth diffusion method and AlamarBlue assay were used to evaluate the antimicrobial activity of EEP, with the estimation of its minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC). The biochemical composition of propolis components was assessed. The mean MIC and MBC values of EEP, in concentrations ranging from 25 mg/mL to 0.025 mg/mL, for the MS and LB were found to be 1.10 mg/mL versus 0.7 mg/mL and 9.01 mg/mL versus 5.91 mg/mL, respectively. The exposure to an extract of Polish propolis affected mutans streptococci and Lactobacillus spp. viability, exhibiting an antibacterial efficacy on mutans streptococci group bacteria and lactobacilli saliva residents, while lactobacilli were more susceptible to EEP. Antibacterial measures containing propolis could be the local agents acting against cariogenic bacteria.

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Figures

Figure 1
Figure 1
The influence of different concentrations of EEP on absorbance changes for MS (n = 17) during 1 hour of EEP activity (OD600—optical density reading at 600 nm).
Figure 2
Figure 2
The influence of different concentrations of EEP on absorbance changes for MS (n = 17) during 4 hours of EEP activity (OD600—optical density reading at 600 nm).
Figure 3
Figure 3
The influence of different concentrations of EEP on absorbance changes for MS (n = 17) during 24 hours of EEP activity (OD600—optical density reading at 600 nm).
Figure 4
Figure 4
The influence of different concentrations of EEP on absorbance changes for Lactobacillus spp. (n = 17) during 1 hour of EEP activity (OD600—optical density reading at 600 nm).
Figure 5
Figure 5
The influence of different concentrations of EEP on absorbance changes for Lactobacillus spp. (n = 17) during 4 hours of EEP activity (OD600—optical density reading at 600 nm).
Figure 6
Figure 6
The influence of different concentrations of EEP on absorbance changes for Lactobacillus spp. (n = 17) during 24 hours of EEP activity (OD600—optical density reading at 600 nm).
Figure 7
Figure 7
Percentage reduction of AlamarBlue at 120 minutes for mutans streptococci (n = 17) treated for 24 hours with ethanol extract of Polish propolis at the concentrations from 12.5 to 0.025 mg/mL.
Figure 8
Figure 8
Total polyphenol and flavonoid content in investigated propolis sample from Poland. Concentrations (% ± standard deviation) of total polyphenols, flavones/flavonols, and flavanones/dihydroflavonols.
See this image and copyright information in PMC

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