Characterization of cholecystokinin octapeptide-stimulated endogenous dopamine release from rat nucleus accumbens in vitro

Abstract

1. The effect of cholecystokinin sulphated octapeptide (CCK8S) on endogenous dopamine release was examined in rat striatal and nucleus accumbens slices, by high performance liquid chromatography (h.p.l.c.) with electrochemical detection. 2. CCK8S was shown to increase dopamine release from slices of nucleus accumbens but not striatum in a dose-dependent manner between 0.1 and 10 microM. 3. Pentagastrin was without effect on dopamine release at doses up to 10 microM. 4. The dopamine release produced in the presence of CCK8S was abolished by preincubation of the slice with 1.0 or 10.0 nM L-364,718 (the CCKA-selective antagonist) while 1 microM L-365,260 (the CCKB-selective antagonist) had no action. 5. These results suggest that the CCK8S-evoked release of dopamine from the nucleus accumbens is mediated by a CCKA-receptor.