High-copy sequences reveal distinct evolution of the rye B chromosome
- PMID: 23614816
- DOI: 10.1111/nph.12289
High-copy sequences reveal distinct evolution of the rye B chromosome
Abstract
B chromosomes (Bs) are supernumerary chromosomes that vary in number among individuals of the same species. Because of their dispensable nature, their non-Mendelian inheritance and their origin from A chromosomes (As), one might assume that Bs followed a different evolutionary pathway from As, this being reflected in differences in their high-copy DNA constitution. We provide detailed insight into the composition and distribution of rye (Secale cereale) B-located high-copy sequences. A- and B-specific high-copy sequences were identified in silico. Mobile elements and satellite sequences were verified by fluorescence in situ hybridization (FISH). Replication was analyzed via EdU incorporation. Although most repeats are similarly distributed along As and Bs, several transposons are either amplified or depleted on the B. An accumulation of B-enriched satellites was found mostly in the nondisjunction control region of the B, which is transcriptionally active and late-replicating. All B-enriched sequences are not unique to the B but are also present in other Secale species, suggesting the origin of the B from As of the same genus. Our findings highlight the differences between As and Bs. Although Bs originated from As, they have since taken a separate evolutionary pathway.
© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.
References
-
- Alfenito MR, Birchler JA. 1993. Molecular characterization of a maize B chromosome centric sequence. Genetics 135: 589-597.
-
- Banaei-Moghaddam AM, Schubert V, Kumke K, Weiβ O, Klemme S, Nagaki K, Macas J, González-Sánchez M, Heredia V, Gómez-Revilla D et al. 2012. Nondisjunction in favor of a chromosome: the mechanism of rye B chromosome drive during pollen mitosis. Plant Cell 24: 4124-4134.
-
- Blunden R, Wilkes TJ, Forster JW, Jimenez MM, Sandery MJ, Karp A, Jones RN. 1993. Identification of the E3900 family, a 2nd family of rye chromosome-B specific repeated sequences. Genome 36: 706-711.
-
- Bugrov AG, Karamysheva TV, Perepelov EA, Elisaphenko EA, Rubtsov DN, Warchalowska-Sliwa E, Tatsuta H, Rubtsov NB. 2007. DNA content of the B chromosomes in grasshopper Podisma kanoi Storozh. (Orthoptera, Acrididae). Chromosome research 15: 315-325.
-
- Carchilan M, Delgado M, Ribeiro T, Costa-Nunes P, Caperta A, Morais-Cecilio L, Jones RN, Viegas W, Houben A. 2007. Transcriptionally active heterochromatin in rye B chromosomes. Plant Cell 19: 1738-1749.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials